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http://dx.doi.org/10.1021/acssynbio.4c00583 | DOI Listing |
J Vet Emerg Crit Care (San Antonio)
August 2025
Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Washington State University, Pullman, Washington, USA.
Objective: To develop a PCV-dependent hemolysis color scale and evaluate its accuracy in predicting supernatant hemoglobin concentration in packed red blood cell (pRBC) products, helping to determine transfusion safety.
Design: Prospective experimental study.
Setting: University veterinary teaching hospital.
Background: Next generation sequencing (NGS) of cell free tumour DNA (ctDNA) provides a snapshot of lymphoma mutations correlating with tumour burden. We evaluated the ability of ctDNA to molecularly profile and track disease burden in patients with aggressive-B-cell lymphoma.
Methods: Patients were prospectively recruited from the 'standard-of-care' clinic based on high-risk clinical features.
bioRxiv
August 2025
New York Genome Center, New York, NY, United States.
Distinguishing real biological variation in the form of single-nucleotide variants (SNVs) from errors is a major challenge for genome sequencing technologies. This is particularly true in settings where SNVs are at low frequency such as cancer detection through liquid biopsy, or human somatic mosaicism. State-of-the-art molecular denoising approaches for DNA sequencing rely on duplex sequencing, where both strands of a single DNA molecule are sequenced to discern true variants from errors arising from single stranded DNA damage.
View Article and Find Full Text PDFJ Control Release
August 2025
Clinical Stem Cell Center, Nanjing Drum Tower Hospital, Clinical Medical College of Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, Jiangsu Province 210009, China; Clinical Stem Cell Center, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School
Inflammatory bowel disease (IBD), including Crohn's disease (CD; Th1/Th17-driven) and ulcerative colitis (Th2-skewed), lacks therapies correcting T-cell imbalance. Current cytokine-focused treatments remain ineffective, while mesenchymal stromal cells (MSCs) therapies are hindered by inherent heterogeneity and challenges related to cell viability maintenance, batch-to-batch consistency, and standardization. This study aimed to (Kaplan, 2015 (1)) identify MSCs subtypes targeting CD pathology, Sebastian and Siegmund (2024) (2) create MSCs-mimicking nanoparticles, and (Li et al.
View Article and Find Full Text PDFBMC Pregnancy Childbirth
August 2025
Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi'an Jiaotong University, No.98, Xiwu Road, Xi'an, Shaanxi, People's Republic of China.