A comparative analysis of beta-cypermethrin nanoemulsion and traditional emulsion dosages on enzyme activities in .

To compare the impact of two pesticide dose forms on ()resistance enzymes.The micro-drop technique was utilized on subcultured , and the metabolic enzyme activity was assessed.Using spectrophotometry, the relative enzyme activities of were determined.Profile analysis was used to compare the enzyme activities of beta-cypermethrin in both nanoemulsion and traditional emulsion forms.Findings: The suppression percentages of the acetylcholinesterase enzyme (AchE), GST, and P450-O demethylases across different pesticide formulations were analyzed using regression equations, yielding  = 31.18, P < 0.001;  = 9.18, P < 0.001; and  = 4.58, P < 0.02.Conclusion: The suppression of AchE, GST, and P450-O demethylase by beta-cypermethrin nanoemulsion was more significant compared to the beta-cypermethrin emulsifiable concentrate.The study concluded that beta-cypermethrin nanoemulsion had a significant impact on insect detoxifying enzymes compared to beta-cypermethrin emulsifiable concentrate due to their numerous advantages.