A near-zero-discharge recirculating aquaculture system with 3D-printed poly (lactic acid) honeycomb as solid carbon.

A novel near-zero-discharge recirculating aquaculture system was successfully set up and ran for six months or above. A uniquely designed and 3D printed poly (lactic acid) (PLA) structure was applied as carbon source. The system achieved over 50 % daily nitrogen removal capability and maintained a low NO-N level of <0.5 mg/L. Steady water quality was observed throughout the experiment period. Microbial distribution was studied and top abundant microorganisms and their general functions in carbon and nitrogen utilization were discussed. Denitrification and L-glutamate formation were identified as two main nitrogen pathways. The cooccurrence network connecting various genera and multiple functions was revealed. Subtilisin was one important PLA degrading enzymes in the system.