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With the rise of global smart city construction, target detection technology plays a crucial role in optimizing urban functions and improving the quality of life. However, existing target detection technologies still have shortcomings in terms of accuracy, real-time performance, and adaptability. To address this challenge, this study proposes an innovative target detection model. Our model adopts the structure of YOLOv8-DSAF, comprising three key modules: depthwise separable convolution (DSConv), dual-path attention gate module (DPAG), and feature enhancement module (FEM). Firstly, DSConv technology optimizes computational complexity, enabling real-time target detection within limited hardware resources. Secondly, the DPAG module introduces a dual-channel attention mechanism, allowing the model to selectively focus on crucial areas, thereby improving detection accuracy in high-dynamic traffic scenarios. Finally, the FEM module highlights crucial features to prevent their loss, further enhancing detection accuracy. Additionally, we propose an Internet of Things smart city framework consisting of four main layers: the application domain, the Internet of Things infrastructure layer, the edge layer, and the cloud layer. The proposed algorithm utilizes the Internet of Things infrastructure layer, edge layer, and cloud layer to collect and process data in real-time, achieving faster response times. Experimental results on the KITTI V and Cityscapes datasets indicate that our model outperforms the YOLOv8 model. This suggests that in complex urban traffic scenarios, our model exhibits superior performance with higher detection accuracy and adaptability. We believe that this innovative model will significantly propel the development of smart cities and advance target detection technology.
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http://dx.doi.org/10.1038/s41598-024-68115-1 | DOI Listing |
J Appl Microbiol
September 2025
Graduate Institute of Medical Sciences, National Defense Medical University, Taipei City 114201, Taiwan (R.O.C.).
Aims: This study aims to develop and evaluate a rapid and high-multiplex pathogen detection method for clinical and food specimens to address the ongoing public health threat of foodborne infections and the limitations of conventional culture-based diagnostics.
Methods And Results: The foodborne bacteria (FBB) assay integrates multiplex PCR, T7 exonuclease hydrolysis, and a suspension bead array to simultaneously detect 16 genes from 13 major foodborne bacteria. Analytical performance was evaluated using reference strains, while diagnostic performance was assessed using clinical and food samples.
FEMS Microbiol Rev
September 2025
CIISA - Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, Lisbon, Portugal.
African Swine Fever (ASF), caused by the highly contagious African swine fever virus (ASFV), poses a significant threat to domestic and wild pigs worldwide. Despite its limited host range and lack of zoonotic potential, ASF has severe socio-economic and environmental consequences. Current control strategies primarily rely on early detection and culling of infected animals, but these measures are insufficient given the rapid spread of the disease.
View Article and Find Full Text PDFJAMA Pediatr
September 2025
Department of Pediatrics and Emergency Medicine, Children's National Hospital, George Washington University, Washington, DC.
Importance: Adolescents account for almost half of the 2.5 million diagnosed sexually transmitted infections in the US annually, and the emergency department functions as the primary source of health care for many adolescents. No recommendations exist for emergency department gonorrhea and chlamydia screening.
View Article and Find Full Text PDFJAMA Intern Med
September 2025
Bayer CC AG, Basel, Switzerland.
Importance: There is an unmet need for long-term, safe, effective, and hormone-free treatments for menopausal symptoms, including vasomotor symptoms (VMS) and sleep disturbances.
Objective: To evaluate the 52-week efficacy and safety of elinzanetant, a dual neurokinin-targeted therapy, for treating moderate to severe VMS associated with menopause.
Design, Setting, And Participants: OASIS-3 was a double-blind, placebo-controlled, randomized phase 3 clinical trial that was conducted at 83 sites in North America and Europe from August 27, 2021, to February 12, 2024, and included postmenopausal women aged 40 to 65 years who were seeking treatment for moderate to severe VMS (no requirement for a minimum number of VMS events per week).
Vet Med Sci
September 2025
Department of Pharmacology and Toxicology, Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet, Bangladesh.
The emergence of antimicrobial resistance (AMR) Escherichia coli in poultry farming is a growing global public health concern, particularly in Bangladesh, where the use of antibiotics remains largely unregulated. This study aimed to determine the prevalence and AMR patterns of E. coli isolated from broiler chickens in Sylhet district of Bangladesh and to investigate the network of coexisting resistance traits among the isolates.
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