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The electroencephalogram (EEG) is crucial for real-time brain physiology research in epilepsy. However, maternal care reliance limits its use in immature rodents. Our "pup-in-cup" setup overcomes this, enabling continuous, uninterrupted video-EEG/electromyogram (EMG) recordings in neonatal rats. This protocol details the steps for video-EEG/EMG system setup, EEG headmount implantation, and recording continuous video-EEG/EMG traces from postnatal days 4-12. For complete details on the use and execution of this protocol, please refer to Choudhary et al..
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http://dx.doi.org/10.1016/j.xpro.2024.103205 | DOI Listing |
STAR Protoc
September 2024
Department of Pediatrics, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada; Alberta Children's Hospital Research Institute, University of Calgary, Calgary, AB, Canada; Hotchkiss Brain Institute, University of Calgary, Calgary, AB, Canada; Department of Clinical Neuroscience, Cu
The electroencephalogram (EEG) is crucial for real-time brain physiology research in epilepsy. However, maternal care reliance limits its use in immature rodents. Our "pup-in-cup" setup overcomes this, enabling continuous, uninterrupted video-EEG/electromyogram (EMG) recordings in neonatal rats.
View Article and Find Full Text PDFEpileptic Disord
June 2023
Swiss Epilepsy Center, Clinic Lengg, Zurich, Switzerland.
Objective: Patients with Unverricht-Lundborg disease/EPM1 develop increasing locomotory disability or ataxia in the course of their disease. To test our hypothesis that negative myoclonus is the reason for this increasing ataxia, we investigated a possible correlation over time.
Methods: In 15 patients with EPM1who were confirmed to have a mutation in the CSTB gene, polygraphic video-EEG-EMG recordings were performed in freely moving or standing patients.
Brain Stimul
March 2021
Department of Neurosurgery, Emory University, USA; Department of Neurology, Emory University, USA. Electronic address:
Background: Narcolepsy type 1 (NT1, narcolepsy with cataplexy) is a disabling neurological disorder caused by loss of excitatory orexin neurons from the hypothalamus and is characterized by decreased motivation, sleep-wake fragmentation, intrusion of rapid-eye-movement sleep (REMS) during wake, and abrupt loss of muscle tone, called cataplexy, in response to sudden emotions.
Objective: We investigated whether subcortical stimulation, analogous to clinical deep brain stimulation (DBS), would ameliorate NT1 using a validated transgenic mouse model with postnatal orexin neuron degeneration.
Methods: Using implanted electrodes in freely behaving mice, the immediate and prolonged effects of DBS were determined upon behavior using continuous video-electroencephalogram-electromyogram (video/EEG/EMG) and locomotor activity, and neural activation in brain sections, using immunohistochemical labeling of the immediate early gene product c-Fos.
Neuroscience
September 2014
Department of Neurology, Johns Hopkins University, Baltimore, MD 21205, USA; Integrated Research Center for Fetal Medicine, Department of Gynecology and Obstetrics, Johns Hopkins University, Baltimore, MD 21205, USA. Electronic address:
Intrauterine infection or inflammation in preterm neonates is a known risk for adverse neurological outcomes, including cognitive, motor and behavioral disabilities. Our previous data suggest that there is acute fetal brain inflammation in a mouse model of intrauterine exposure to lipopolysaccharides (LPS). We hypothesized that the in utero inflammation induced by LPS produces long-term electroencephalogram (EEG) biomarkers of neurodegeneration in the exposed mice that could be determined by using continuous quantitative video/EEG/electromyogram (EMG) analyses.
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