Protocol for continuous video-EEG/EMG recording to study brain function in neonatal rats.

STAR Protoc

Department of Pediatrics, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada; Alberta Children's Hospital Research Institute, University of Calgary, Calgary, AB, Canada; Hotchkiss Brain Institute, University of Calgary, Calgary, AB, Canada; Department of Clinical Neuroscience, Cu

Published: September 2024


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Article Abstract

The electroencephalogram (EEG) is crucial for real-time brain physiology research in epilepsy. However, maternal care reliance limits its use in immature rodents. Our "pup-in-cup" setup overcomes this, enabling continuous, uninterrupted video-EEG/electromyogram (EMG) recordings in neonatal rats. This protocol details the steps for video-EEG/EMG system setup, EEG headmount implantation, and recording continuous video-EEG/EMG traces from postnatal days 4-12. For complete details on the use and execution of this protocol, please refer to Choudhary et al..

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11315175PMC
http://dx.doi.org/10.1016/j.xpro.2024.103205DOI Listing

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Protocol for continuous video-EEG/EMG recording to study brain function in neonatal rats.

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