Genome-Driven Discovery of Antiviral Atralabdans A-C from the Soil-Dwelling .

Heterologous expression of an terpenoid gene cluster derived from Gö66 in J1074 led to the discovery of three novel labdane diterpenoids featuring an unprecedented 6/6/5-fused tricyclic skeleton, designated as atralabdans A-C (-), along with a known compound, labdanmycin A. Compounds - were identified through extensive spectroscopic analysis, including NMR calculations with DP4+ probability analysis, and a comparative assessment of experimental and theoretical electronic circular dichroism (ECD) spectra. A plausible biosynthetic pathway for these compounds was proposed. Compounds - exhibited inhibitory activity against the human neurotropic coxsackievirus B3 (CVB3); was the most potent, surpassing the positive control ribavirin with a higher therapeutic index.