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Glucuronoxylomannan (GXM) is the principal capsular component in the Cryptococcus genus. This complex polysaccharide participates in numerous events related to the physiology and pathogenesis of Cryptococcus, which highlights the importance of establishing methods for its isolation and analysis. Conventional methods for GXM isolation have been extensively discussed in the literature. In this chapter, we describe two fast methods for obtaining extracellular fractions enriched with cryptococcal GXM.
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http://dx.doi.org/10.1007/978-1-0716-3722-7_24 | DOI Listing |
Nat Metab
September 2025
Cellular and Molecular Physiology Department, Yale School of Medicine, New Haven, CT, USA.
The essential cofactor coenzyme A (CoASH) and its thioester derivatives (acyl-CoAs) have pivotal roles in cellular metabolism. However, the mechanism by which different acyl-CoAs are accurately partitioned into different subcellular compartments to support site-specific reactions, and the physiological impact of such compartmentalization, remain poorly understood. Here, we report an optimized liquid chromatography-mass spectrometry-based pan-chain acyl-CoA extraction and profiling method that enables a robust detection of 33 cellular and 23 mitochondrial acyl-CoAs from cultured human cells.
View Article and Find Full Text PDFPlant J
September 2025
Department of Biological Sciences, Royal Holloway University of London, Egham, Surrey, TW20 0EX, UK.
Plastoglobuli (PG) are plant lipoprotein compartments, present in plastid organelles. They are involved in the formation and/or storage of lipophilic metabolites. FIBRILLINs (FBNs) are one of the main PG-associated proteins and are particularly abundant in carotenoid-enriched chromoplasts found in ripe fruits and flowers.
View Article and Find Full Text PDFPLoS One
September 2025
Department of Cardiology Ullevaal, Oslo University Hospital, Oslo, Norway.
Background: The gut microbiota produces numerous metabolites that can enter the circulation and exert effects outside the gut. Several studies have reported altered gut microbiota composition and circulating metabolites in patients with chronic heart failure (HF) compared to healthy controls. Limited data is available on the interplay between dysbiotic features of the gut microbiota and altered circulating metabolites in HF patients.
View Article and Find Full Text PDFParasite Immunol
September 2025
Department of Parasitology, Leiden University Medical Center, Leiden, the Netherlands.
Schistosome parasites are known to modulate host immune responses, which is achieved in part through the release of excretory/secretory (ES) products, including extracellular vesicles (EVs). During chronic schistosomiasis, increased regulatory responses are found, which include enhanced IL-10 production by B (Breg) cells. ES products from schistosome eggs are able to induce IL-10 production by B cells.
View Article and Find Full Text PDFWater Res
August 2025
School of Materials and Energy, University of Electronic Science and Technology of China, 610054 Chengdu, China; Sichuan Province Engineering Technology Research Center of Novel CN Polymeric Materials, Chengdu, China. Electronic address:
The scalable fabrication of high performance dyes desalination loose nanofiltration (LNF) membrane through facile thermal annealing remains challenging due to the susceptible pore collapse. Herein, we have developed a metal ion mediated sub-Tg thermal crosslinking protocol, which can convert the phase inverted reactive polymeric ultrafiltration substrate into LNF membrane showing high permselectivity as well as resistance to both extremely acid and alkaline solution. The original ultrafiltration substrate was composed of scalable-produced reactive polyarylene ether amidoxime (PEA) that was pre-crosslinked with ferric ions.
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