98%
921
2 minutes
20
Download full-text PDF |
Source |
---|---|
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11631692 | PMC |
http://dx.doi.org/10.1136/gutjnl-2024-332818 | DOI Listing |
J Natl Compr Canc Netw
June 2025
1Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA.
Over the past 20 years, long-term cure rates in T-lymphoblastic leukemia/lymphoma (T-ALL) have improved from 60% to 65% to >80%, largely due to the intensification of chemotherapy regimens. In B-lymphoblastic leukemia/lymphoma (B-ALL), outcomes have been improved through risk stratification, integrating clinical and demographic features, response to therapy, and disease biology to identify patients more or less likely to respond to conventional treatment, allowing for the use of regimens adapted to relative risk. Unfortunately, in T-ALL, few features have been identified that can independently predict the risk of poor outcomes beyond response to therapy.
View Article and Find Full Text PDFSci Rep
May 2025
Department of Molecular and Cellular Biology, University of California, Davis, 95616, USA.
The wide diversity of organisms in which introns stimulate gene expression suggests that this is an ancient phenomenon. However, the mechanisms through which introns boost expression remain poorly understood, and the degree the which the action of introns is evolutionarily conserved is unknown. Here we compared the effect on expression of introns at different positions and tested ten different introns at the same location in a reporter gene in single-copy transgenic nematodes.
View Article and Find Full Text PDFBiochim Biophys Acta Gene Regul Mech
June 2025
Division of Applied Chemistry, Graduate School of Sciences and Technology for Innovation, Yamaguchi University, 2-16-1 Tokiwadai, Ube 755-8611, Japan; Research Center for Thermotolerant Microbial Resources, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8315, Japan; Yamaguchi University Biomedi
Introns can enhance gene expression, a phenomenon called intron-mediated enhancement (IME). Previously proposed IME mechanisms do not sufficiently explain the variability in enhancement levels, suggesting that IME mechanism has not been fully understood. A comprehensive screening of genes involved in IME can provide valuable insights.
View Article and Find Full Text PDFFront Plant Sci
March 2025
Centre for Research in Agricultural Genomics (CRAG), CSIC-IRTA-UAB-UB, Barcelona, Spain.
The Modular Cloning (MoClo) toolkit allows for straightforward and flexible construction of genetic modules for gene expression in the microalgal model species, fostering developments in algal biotechnology. Efficiently expressing transgenes from the nuclear genome of requires the proper insertion of introns throughout the respective gene, as it can substantially enhance the gene expression. To facilitate synthetic biology approaches in this microalga, we developed a novel strategy for intron insertion into synthetic DNA fragments.
View Article and Find Full Text PDFNucleic Acids Res
February 2025
Department of Biology, Massachusetts Institute of Technology, Cambridge MA 02139, United States.
Spliceosomal introns are a ubiquitous feature of eukaryotic genes, whose presence often boosts the expression of their host gene, a phenomenon known as intron-mediated enhancement (IME). IME has been noted across diverse genes and organisms but remains mysterious in many respects. For example, how does intron sequence affect the magnitude of IME? In this study, we performed a massively parallel reporter assay (MPRA) to assess the effect of varying intron sequence on gene expression in a high-throughput manner, in human cells, using tens of thousands of synthetic introns with natural splice sites and randomized internal sequence.
View Article and Find Full Text PDF