Article Synopsis
- Non-thermal atmospheric-pressure plasma (NTAPP) shows promise in promoting fracture healing, particularly in non-union fractures, which are challenging to treat clinically.
- In vivo studies on rats indicated that while NTAPP did not significantly affect normal fracture healing, it significantly improved healing in non-union fracture models, confirmed by histological and biomechanical assessments.
- In vitro experiments with bone precursor cells revealed that NTAPP enhances osteoblastic differentiation without decreasing cell proliferation, suggesting its potential as a novel treatment for bone fractures.
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Article Abstract
Non-thermal atmospheric-pressure plasma (NTAPP) is attracting widespread interest for use in medical applications. The tissue repair capacity of NTAPP has been reported in various fields; however, little is known about its effect on fracture healing. Non-union or delayed union after a fracture is a clinical challenge. In this study, we aimed to investigate how NTAPP irradiation promotes fracture healing in a non-union fracture model and its underlying mechanism, in vitro and in vivo. For the in vivo study, we created normal and non-union fracture models in LEW/SsNSlc rats to investigate the effects of NTAPP. To create a fracture, a transverse osteotomy was performed in the middle of the femoral shaft. To induce the non-union fracture model, the periosteum surrounding the fracture site was cauterized after a normal fracture model was created. The normal fracture model showed no significant difference in bone healing between the control and NTAPP-treated groups. The non-union fracture model demonstrated that the NTAPP-treated group showed consistent improvement in fracture healing. Histological and biomechanical assessments confirmed the fracture healing. The in vitro study using pre-osteoblastic MC3T3-E1 cells demonstrated that NTAPP irradiation under specific conditions did not reduce cell proliferation but did enhance osteoblastic differentiation. Overall, these results suggest that NTAPP is a novel approach to the treatment of bone fractures.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11020618 | PMC |
| http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0298086 | PLOS |
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