Article Synopsis
- Scientists are developing a new method called FixNCut to help study tiny cells better!
- This method helps keep the cells' important information safe during processing, which can make research results more reliable!
- FixNCut can be used with different types of studies, so it’s a helpful tool for looking at cells from humans and mice!
Video Abstracts
Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
The use of single-cell technologies for clinical applications requires disconnecting sampling from downstream processing steps. Early sample preservation can further increase robustness and reproducibility by avoiding artifacts introduced during specimen handling. We present FixNCut, a methodology for the reversible fixation of tissue followed by dissociation that overcomes current limitations. We applied FixNCut to human and mouse tissues to demonstrate the preservation of RNA integrity, sequencing library complexity, and cellular composition, while diminishing stress-related artifacts. Besides single-cell RNA sequencing, FixNCut is compatible with multiple single-cell and spatial technologies, making it a versatile tool for robust and flexible study designs.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10979608 | PMC |
| http://dx.doi.org/10.1186/s13059-024-03219-5 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
FixNCut: A Practical Guide to Sample Preservation by Reversible Fixation for Single Cell Assays.
Bio Protoc
September 2024
Adelaide Centre for Epigenetics (ACE), University of Adelaide, Adelaide, South Australia, Australia.
The quality of standard single-cell experiments often depends on the immediate processing of cells or tissues post-harvest to preserve fragile and vulnerable cell populations, unless the samples are adequately fixed and stored. Despite the recent rise in popularity of probe-based and aldehyde-fixed RNA assays, these methods face limitations in species and target availability and are not suitable for immunoprofiling or assessing chromatin accessibility. Recently, a reversible fixation strategy known as FixNCut has been successfully deployed to separate sampling from downstream applications in a reproducible and robust manner, avoiding stress or necrosis-related artifacts.
View Article and Find Full Text PDFFixNCut: single-cell genomics through reversible tissue fixation and dissociation.
Genome Biol
March 2024
Adelaide Centre for Epigenetics (ACE), University of Adelaide, Adelaide, South Australia, Australia.
Article Synopsis
- Scientists are developing a new method called FixNCut to help study tiny cells better!
- This method helps keep the cells' important information safe during processing, which can make research results more reliable!
- FixNCut can be used with different types of studies, so it’s a helpful tool for looking at cells from humans and mice!
Novel Approach for Pancreas Transcriptomics Reveals the Cellular Landscape in Homeostasis and Acute Pancreatitis.
Gastroenterology
June 2024
Biological and Biomedical Sciences Program, Harvard Medical School, Boston, Massachusetts; Health Sciences & Technology Program, Harvard-MIT, Boston, Massachusetts; Genetics Division, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts; Dana-Farber Cancer Institute, Boston, M
Background & Aims: Acinar cells produce digestive enzymes that impede transcriptomic characterization of the exocrine pancreas. Thus, single-cell RNA-sequencing studies of the pancreas underrepresent acinar cells relative to histological expectations, and a robust approach to capture pancreatic cell responses in disease states is needed. We sought to innovate a method that overcomes these challenges to accelerate study of the pancreas in health and disease.
View Article and Find Full Text PDF