Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
This protocol describes the use of the Spectradyne nCS1 instrument to measure the particles per mL concentration and size of nanoparticles. The Spectradyne nCS1 is a particle-analyzing instrument that uses microfluidic resistive pulse sensing, rather than optical measurements, to determine the size and concentration of samples. The size and concentration of a sample are determined by measuring the changes in voltage as particles travel through a nano-constriction in the microfluidic cartridge. This method also has the advantage over optical techniques in that measurements are not dependent on the type of material being measured (e.g., refractive index of the sample itself is not needed for accurate analysis), and only microliters (typically 5 μL) of a sample are needed for analysis.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/978-1-0716-3786-9_5 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
[Organ and species identification of liver microparticles using forensic cytology and quantitative enzyme-linked immunosorbent assay methods].
Sud Med Ekspert
January 2025
Bureau of Forensic Medical Expertise, Saint Petersburg, Russia.
Objective: To establish organ affiliation of liver microparticles using forensic cytological method based on hepatocytes' morphological characteristics and to determine their species belonging according to the human IgG using a quantitative enzyme-linked immunosorbent assay (ELISA).
Material And Methods: Previously dried microparticles (from 0.2×0.
Tetrandrine has protective role in myocardial ischemia/reperfusion injury via the TRPV2/Ca/calcineurin/NFAT axis.
Herz
September 2025
Department of Cardiology, The Third Clinical College of Wenzhou Medical University, 326000, Wenzhou, Zhejiang, China.
Background: The protective function of the tetrandrine (TET)-mediated transient receptor potential vanilloid 2 (TRPV2) channel in myocardial ischemia/reperfusion injury (MI/RI) has been established in numerous investigations. The objective of the current study was to explain how TRPV2 further modulates downstream factors to influence the progression of MI/RI.
Methods: To this end, an MI/RI model in rats and a hypoxia-reoxygenation (H/R) cell model in H9c2 cells were constructed.
Genomic insights into multidrug-resistant extraintestinal pathogenic Escherichia coli O4: H12 harboring mcr-5 and blaSHV-12 on conjugative plasmids isolated from chicken meat in Japan.
J Appl Microbiol
September 2025
Laboratory of Food Microbiology and Hygiene, Graduate School of Integrated Sciences for Life, Hiroshima University, 1-4-4 Kagamiyama, Higashihiroshima 739-8528, Japan.
Aims: This study aims to investigate the genomic profile of a multidrug-resistant Escherichia coli strain, 160-11H1, co-carrying an extended-spectrum β-lactamase (ESBL) and the plasmid-mediated mobile colistin resistance gene, mcr-5.
Methods And Results: The entire genome of the strain was sequenced using Illumina MiSeq and Oxford Nanopore platforms, and de novo assembly was performed using Unicycler. The genome size was 5 031,330 bp and comprised 5 140 coding sequences.
Visualizing NEK9 in action: aptamer-based fluorescent probes for real-time live-cell imaging.
Nucleosides Nucleotides Nucleic Acids
September 2025
School of Basic Medical Sciences, Yan'an University, Yan'an, China.
Live-cell imaging of intracellular proteins enables real-time observation of protein dynamics under near-physiological conditions, providing pivotal insights for both fundamental life science research and medical applications. However, due to limitations such as poor probe permeability and cytotoxicity associated with conventional antibody-based or genetically encoded labeling techniques, live-cell imaging remains a significant challenging. To address these limitations, here in this study, we developed and rigorously validated a novel aptamer-based fluorescent probe for real-time imaging of NEK9 kinase in living cells.
View Article and Find Full Text PDFEnhancing Antifungal Efficacy and Stability of Nystatin Liposomes Through Chitosan and Alginate Layer-by-Layer Coating: In vitro Studies Against .
Int J Nanomedicine
September 2025
Department of Pharmaceutics and Pharmaceutical Technology, Universitas Padjadjaran, Sumedang, West Java, 45363, Indonesia.
Background: Candidiasis, predominantly caused by , poses a significant global health challenge, especially in tropical regions. Nystatin is a potent antifungal agent that is hindered by its low solubility and permeability, limiting its clinical efficacy.
Methods: This study aimed to investigate the potential of a layer-by-layer (LBL) coating system, employing chitosan and alginate, to improve the stability, entrapment efficiency (%EE), and antifungal efficacy of nystatin-loaded liposomes against Candida albicans.