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The normal probability density function (PDF) is widely used in parameter estimation in the modeling of dynamic systems, assuming that the random variables are distributed at infinite intervals. However, in practice, these random variables are usually distributed in a finite region confined by the physical process and engineering practice. In this study, we address this issue through the application of truncated normal PDF. This method avoids a non-differentiable problem inherited in the truncated normal PDF at the truncation points, a limitation that can limit the use of analytical methods (e.g., Gaussian approximation). A data assimilation method with the derived formula is proposed to describe the probability of parameter and measurement noise in the truncated space. In application to a water distribution system (WDS), the proposed method leads to estimating nodal water demand and hydraulic pressure key to hydraulic and water quality model simulations. Application results to a hypothetical and a large field WDS clearly show the superiority of the proposed method in parameter estimation for WDS simulations. This improvement is essential for developing real-time hydraulic and water quality simulation and process control in field applications when the parameter and measurement noise are distributed in the finite region.
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http://dx.doi.org/10.2166/hydro.2023.250 | DOI Listing |
Elife
September 2025
Department of Chemistry, University of Massachusetts, Amherst, United States.
Voltage-dependence gating of ion channels underlies numerous physiological and pathophysiological processes, and disruption of normal voltage gating is the cause of many channelopathies. Here, long timescale atomistic simulations were performed to directly probe voltage-induced gating transitions of the big potassium (BK) channels, where the voltage sensor domain (VSD) movement has been suggested to be distinct from that of canonical Kv channels but remains poorly understood. Using a Core-MT construct without the gating ring, multiple voltage activation transitions were observed at 750 mV, allowing detailed analysis of the activated state of BK VSD and key mechanistic features.
View Article and Find Full Text PDFCarbohydr Polym
November 2025
School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China; Qingdao Marine Science and Technology Center, Qingdao 266237, China; Key Laboratory of Marine Drugs, Ministry of Education, Qingdao 266003, China; Shandong Key Laboratory of Glycoscience and Glycotherapeutics, Qingdao
Hyaluronic acid (HA), a linear glycosaminoglycan, serves as a key structural constituent of extracellular matrices, participating in diverse biological processes across both normal physiological and pathological contexts. While the gut microbiota exerts a pivotal influence on HA utilization within the human body, current scientific literature indicates a limited understanding of the molecular mechanisms underlying this interaction. In this study, a gut bacterium Enterococcus faecalis F1221 has been isolated, which demonstrated the ability to degrade HA.
View Article and Find Full Text PDFIn most animals, oocyte polarity establishes the embryonic body plan by asymmetrically localizing axis-determining transcripts. These transcripts first localize in and zebrafish oocytes to the Balbiani body (Bb), a large membrane-less organelle conserved from insects to humans. The Bb is transient, disassembling and anchoring at one pole the axis-determining transcripts that establish the vegetal pole of the oocyte.
View Article and Find Full Text PDFStem Cell Res
August 2025
Berlin Institute of Health at Charité - Universitätsmedizin Berlin, Core Unit pluripotent Stem Cells and Organoids, Berlin 13353, Germany. Electronic address:
We generated the human induced pluripotent stem cell (iPSC) line BIHi261-A from dermal fibroblasts of a patient with severe early-onset obesity caused by a homozygous truncating mutation in the POMC gene (W84X). Reprogramming was performed using a non-integrating, RNA-based vector expressing key pluripotency factors. The resulting iPSC line exhibited typical morphology, expressed markers of undifferentiated cells, maintained a normal karyotype, and demonstrated the capacity to differentiate into cell types of all three germ layers.
View Article and Find Full Text PDFGenes Genomics
September 2025
Infectious Diseases Department, The Affiliated Women and Children's Hospital of Ningbo University, Ningbo, 315000, Zhejiang, China.
Background: Dystrophinopathy is severe X-linked recessive muscle disease caused by mutations in DMD gene. There is an increasing number of deep intronic variants in DMD gene, and understanding the pathogenic mechanisms of intronic variants can help the diagnosis and treatment of patients with DMD.
Objective: To identify two novel splice site variants in two families affected with Dystrophinopathy.