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Mosquito-borne diseases especially, dengue is gaining currency nowadays in Pakistan. As there is no approved dengue vaccine available worldwide, prevention and control of vector is the only solution amid prevailing circumstances. The present study is a maiden attempt to screen indoor and outdoor breeding containers for the presence of Aedes () aegypti larvae from selected study districts of Punjab, Pakistan i.e., Dera Ghazi Khan (DG Khan), Chakwal, and Faisalabad. A total of 384 houses from each study districts were surveyed for a calendar year. Mosquito larvae were collected, preserved, and identified using standard taxonomic keys. House Index (HI), Container Index (CI), and Breteau Index (BI) were estimated. Chi-square analysis was applied to calculate the association between larvae and breeding containers. Chakwal was identified with the highest values of Stegomyia indices (HI = 46.61 %, BI = 91.67 %, and CI = 15.28 %) followed by Faisalabad (HI = 34.11 %, BI = 68.75 % and, CI = 13.04 %) and DG Khan (HI = 28.39 %, BI = 68.23 % and, CI = 11.29 %). Earthen jars, tree holes, and water tanks were found significantly (p < 0.05) associated with the abundance of larvae irrespective of the geographical location. However, flower tubs and plastic buckets were found significantly (p < 0.05) associated in Faisalabad and Chakwal while, tyres and plastic bottles were found associated (p < 0.05) with the abundance of larvae in Faisalabad and DG Khan. These findings will help the stakeholders to devise appropriate preventive measures in combating the risk of dengue transmission.
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http://dx.doi.org/10.1016/j.sjbs.2023.103919 | DOI Listing |
Acta Parasitol
August 2025
Departamento de Zoologia, Universidade Federal do Paraná, CP 19020, Curitiba, Paraná, 81531-980, Brazil.
Objective: Aedes (Stegomyia) albopictus, commonly known as the Asian Tiger mosquito, is native to tropical and subtropical regions of South and East Asia and has rapidly spread globally. Due to its role as a vector of several medically important arboviruses, understanding its genetic diversity and dispersal patterns is crucial for effective disease control. This study aimed to evaluate the genetic structure insights of Ae.
View Article and Find Full Text PDFPLoS Negl Trop Dis
July 2025
Hainan General Hospital, Hainan Affiliated Hospital of Hainan Medical University, Haikou, Hainan, China.
Background: Effective dengue management hinges on targeting key vector breeding sites and understanding transmission risks. Despite recurring outbreaks in Pakistan's Khyber Pakhtunkhwa (KP) Province since 2013, comprehensive entomological and virological data remain scarce. This study identified key larval-based indicators (habitats, Stegomyia indices), mosquito species composition, and dengue virus (DENV) infection rates in Aedes mosquitoes, evaluating their contributions to outbreak risk.
View Article and Find Full Text PDFPLoS Negl Trop Dis
July 2025
Laboratoire de Biologie et Santé, UFR Biosciences, Université Félix Houphouët-Boigny, Abidjan, Côte d'Ivoire.
Background: Over the last ten years, there has been an upsurge of dengue outbreaks in Côte d'Ivoire, with Aedes aegypti as the principal vector. The intensification of farming activities and the living conditions of local populations could lead to a proliferation of artificial breeding sites for Ae. aegypti, which would increase the risk of dengue transmission in rural areas.
View Article and Find Full Text PDFJ Vector Borne Dis
July 2025
ICMR-Regional Medical Research Centre, Sri Vijaya Puram, Andaman & Nicobar Islands, India.
Background Objectives: The breeding potential of vector mosquitoes of dengue and chikungunya viruses in and around airport and seaport of Sri Vijaya Puram (Port Blair), Andaman & Nicobar Islands was explored.
Methods: The survey was carried out for two years during the months of March and April. All the water holding containers in the residential and non-residential areas of airport and seaport were surveyed for Aedes immatures.
Plants (Basel)
April 2025
Grupo de Investigación Bionat, Facultad de Ciencias Exactas y Naturales, Universidad de Caldas, Manizales 170001, Caldas, Colombia.
Synthetic insecticides are widely used against mosquitoes, but misuse has led to environmental and health concerns. Plant-derived alternatives, such as essential oils, seem to offer a safer option, minimizing these problems without compromising efficacy. In this study, we evaluated the essential oil from (Kunth) A.
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