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Coffee cherries contain a crucial flavor-precursor and chemical substances influencing roasted bean quality, yet limited knowledge exists on metabolite changes during cherry ripening. Our study identified 1078 metabolites, revealing 46 core differential metabolites using a KEGG pathway analysis. At the GF vs. ROF stage, amino acid synthesis dominated; ROF vs. BRF featured nucleotide catabolism; BRF vs. PRF exhibited glycoside and flavonoid synthesis; and PRF vs. PBF involved secondary metabolite synthesis and catabolism. The PRF stage emerged as the optimal cherry-harvesting period. A correlation analysis identified core differential metabolites strongly linked to taste indicators, suggesting their potential as taste markers. Notably, nucleotides and derivatives exhibited significant negative correlations with glycosides and flavonoids during ripening. This research systematically analyzed flavor and active substances in green coffee beans during cherry ripening, offering valuable insights into substance formation in L.
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http://dx.doi.org/10.3390/molecules29010180 | DOI Listing |
Mol Biomed
September 2025
National Key Laboratory of Immunity and Inflammation & Institute of Immunology, College of Basic Medical Sciences, Naval Medical University, Shanghai, 200433, China.
Dendritic cells (DCs) play a central role in coordinating immune responses by linking innate and adaptive immunity through their exceptional antigen-presenting capabilities. Recent studies reveal that metabolic reprogramming-especially pathways involving acetyl-coenzyme A (acetyl-CoA)-critically influences DC function in both physiological and pathological contexts. This review consolidates current knowledge on how environmental factors, tumor-derived signals, and intrinsic metabolic pathways collectively regulate DC development, subset differentiation, and functional adaptability.
View Article and Find Full Text PDFFEBS Open Bio
September 2025
Department of Metabolic Disease Research, Institute of Experimental Endocrinology, Biomedical Research Center, Slovak Academy of Sciences, Bratislava, Slovakia.
Electrical pulse stimulation (EPS) represents a useful tool to study exercise-related adaptations of muscle cells in vitro. Here, we examine the metabolic and secretory response of primary human muscle cells from metabolically healthy individuals to the EPS protocol reflecting the episodic nature of real-life exercise training. This intermittent EPS protocol alternates high-frequency stimulation periods with low-frequency resting periods.
View Article and Find Full Text PDFOrthod Craniofac Res
September 2025
Department of Orthodontics, College of Dentistry, University of Florida, Gainesville, Florida, USA.
Objective(s): In this pilot study, exosomes from saliva were isolated and tested for the presence of metabolomic biomarkers for physiological external root resorption and/or pathological alveolar bone resorption.
Settings And Sample Population: Saliva samples of 20 individuals in the mixed dentition stage of dental development.
Materials And Methods: Saliva was obtained from healthy control children with resorbing primary teeth or children with localised aggressive periodontitis (LAP) showing alveolar bone loss but little root resorption.
Food Res Int
November 2025
Department of Food and Drug, University of Parma, Viale Parco Area delle Scienze, 43124 Parma, Italy; Institute of Biophysics, National Research Council (CNR), Via Ugo La Malfa 153, 90146 Palermo, Italy.
The hop plant is gaining interest in the food, pharmaceutical, and cosmetics industries due to its abundance of secondary metabolites. However, branches and leaves, despite their antioxidant potential, are typically discarded. To valorize these components as functional ingredients they were dried, milled into hop powder (HP), and used to enrich bread.
View Article and Find Full Text PDFFood Res Int
November 2025
School of Life Sciences, Mianyang Normal University, Mianyang City, Sichuan Province 621000, China. Electronic address:
As a dual-purpose medicinal and edible plant, Ocimum species offer significant pharmacological potential through their alkaloid metabolites. This study comprehensively analyzed alkaloid profiles in 10 Ocimum accessions using ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), integrated with network pharmacology, molecular docking, and transcriptome sequencing to elucidate key pharmacological targets, therapeutic potentials, and biosynthetic pathways. We identified 191 alkaloids categorized into eight classes, with phenolamine and plumerane alkaloids predominating.
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