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Article Abstract
Certain sets of genes are derived from gene duplication and share substantial sequence similarity in , presenting a significant challenge in determining the specific roles of each gene and their collective impact on cellular processes. Here, we show that a collection of genes can be disrupted in a single animal via multiple rounds of CRISPR/Cas9 mediated genome editing. We found that up to three genes can be simultaneously disrupted in a single editing event with high efficiency. Our approach offers an opportunity to explore the genetic interaction and molecular underpinning of gene clusters with redundant function.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10685264 | PMC |
| http://dx.doi.org/10.17912/micropub.biology.000898 | DOI Listing |
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