Mutations Associated with Distant Metastasis and Mutations Associated with Poor Tumor Differentiation in Colorectal Cancer.

Background: The occurrence, progression, and prognosis of colorectal cancer (CRC) are regulated by EGFR-mediated signaling pathways. However, the relationship between the core genes (///) status in the signaling pathways and clinicopathological characteristics of CRC patients in Hakka population remains controversial.

Methods: Patients were genotyped for (codons 12, 13, 61, 117, and 146), (codons 12, 61, 117, and 146), (codons 600), and (codons 542, 545 and 1047) mutations. Clinical records were collected, and clinicopathological characteristic associations were analyzed together with mutations of studied genes.

Results: Four hundred and eight patients (256 men and 152 women) were included in the analysis. At least one mutation in the four genes was detected in 216 (52.9%) patients, while none was detected in 192 (47.1%) patients. , and mutation status were detected in 190 (46.6%), 11 (2.7%), 10 (2.5%), 34 (8.3%) samples, respectively. exon 2 had the highest proportion (62.5%). Age, tumor site, tumor size, lymphovascular invasion, and perineural invasion were not associated with gene mutations. mutations (adjusted OR 1.675, 95% CI 1.017-2.760, =0.043) and mutations (adjusted OR 5.183, 95% CI 1.239-21.687, =0.024) appeared more frequently in patients with distant metastasis. mutations (adjusted OR 7.224, 95% CI 1.356-38.488, =0.021) and mutations (adjusted OR 3.811, 95% CI 1.268-11.455, =0.017) associated with poorly differentiated tumor.

Conclusion: mutations are associated with distant metastasis and mutations are associated with poor tumor differentiation in CRC. And the results provided a better understanding between clinicopathological characteristics and gene mutations in CRC patients.