NCFM and Lp-115 inhibit colonization and gastric inflammation in a murine model.

Purpose: To determine the role of Lactobacillus strains and their combinations in inhibiting the colonization of and gastric mucosa inflammation.

Methods: Human gastric adenocarcinoma AGS cells were incubated with H. pylori and six probiotic strains ( NCFM, La-14, Lp-115, Lpc-37, Lr-32, and GG) and the adhesion ability of in different combinations was evaluated by fluorescence microscopy and urease activity assay. Male C57BL/6 mice were randomly divided into five groups (uninfected, , +NCFM, +Lp-115, and +NCFM+Lp-115) and treated with two lactobacilli strains (NCFM and Lp-115) for six weeks. colonization and tissue inflammation statuses were determined by rapid urease test, Hematoxylin-Eosin (HE) staining, immunohistochemistry, and qRT-PCR and ELISA.

Results: NCFM, La-14, Lp-115, Lpc-37, Lr-32, and GG reduced adhesion and inflammation caused by infection in AGS cells and mice. Among all probiotics NCFM and , Lp-115 showed significant effects on the eradication and reduction of inflammation and . Compared with the infection group, the mRNA and protein expression levels of IL-8 and TNF-α in the six intervention groups were significantly reduced. The changes in the urease activity ( and ) for 1-7h in each group showed that NCFM, La-14, Lp-115, and GG effectively reduced the colonization of . We observed a higher ratio of lymphocyte and plasma cell infiltration into the lamina propria of the gastric mucosa and neutrophil infiltration in +NCFM+Lp-115 mice. The infiltration of inflammatory cells in lamina propria of the gastric mucosa was reduced in the +NCFM+Lp-115 group. Additionally, the expression of IFN-γ was decreased significantly in the NCFM and Lp-115 treated C57BL/6 mice.

Conclusions: NCFM and Lp-115 can reduce the adhesion of and inhibit the gastric inflammatory response caused by infection.