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Article Abstract
Loss-of-function genetic tools are widely applied for validating therapeutic targets, but their utility remains limited by incomplete on- and uncontrolled off-target effects. We describe artificial RNA interference (ARTi) based on synthetic, ultra-potent, off-target-free shRNAs that enable efficient and inducible suppression of any gene upon introduction of a synthetic target sequence into non-coding transcript regions. ARTi establishes a scalable loss-of-function tool with full control over on- and off-target effects.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10409502 | PMC |
| http://dx.doi.org/10.7554/eLife.84792 | DOI Listing |
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