Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Background: Khapra beetle (Dermestidae: Trogoderma granarium Everts, 1898) is an internationally significant pest of grain crops and stored grain products. Wheat germ traps, routinely used in surveillance sampling of Khapra beetle provide feed-substrates used by the pest throughout its life cycle. However, Khapra beetle larvae, eggs and other traces of the pest, such as larval frass and exuviae, in wheat germ traps are difficult to sort and taxonomically identify. Additionally, high levels of polysaccharides in wheat germ can inhibit PCR based molecular detection of this pest captured in the traps.
Methods And Results: We have developed a sensitive and low-cost protocol for extracting trace levels of Khapra beetle DNA from an entire wheat germ trap. Overnight digestion of entire trap contents in 6 mL of ATL buffer, followed by a 40 min lysis step was optimal for DNA extraction. Paired with reported qPCR assays, this protocol allows the detection of a few hairs of T. granarium in a typical 2-gram wheat germ trap.
Conclusion: This DNA extraction protocol makes it possible to perform a more rapid identification of the pest following wheat germ sample collection. The protocol has potential to improve international efforts for Khapra beetle surveillance.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10520210 | PMC |
| http://dx.doi.org/10.1007/s11033-023-08673-1 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Nutritional supplement containing a nuclear fraction of bovine thymus gland increases the circulating levels of spermidine.
PLoS One
September 2025
Nutrition Innovation Center, Standard Process Inc., Kannapolis, North Carolina, United States of America.
Polyamines (PAs), including spermidine, spermine and their precursor, putrescine, are ubiquitous molecules that are vital for a variety of physiological processes. Recently, PAs gained research attention because of their roles in promoting longevity and preventing age-related diseases. Circulating and tissue levels of PAs appear to decline with age, while higher intake of PAs in humans is correlated with better health during aging.
View Article and Find Full Text PDFFunctional inhibition of wheat germ agglutinin by glycodendrimers: Interplay of affinity, architecture, and temperature.
Carbohydr Res
September 2025
Area for Molecular Function, Division of Material Science, Graduate School of Science and Engineering, Saitama University, Sakura, Saitama, 338-8570, Japan; Medical Innovation Research Unit (MiU), Advanced Institute of Innovative Technology (AIIT), Saitama University, Sakura, Saitama, 338-8570, Japa
Multivalent interactions between lectins and glycans are crucial for biological recognition; however, predicting functional inhibition based on binding affinity remains challenging. Herein, we investigated a series of structurally defined N-acetylglucosamine (GlcNAc)-functionalized dendrimers (1a-1c and 2a-2c) to examine how spatial orientation and temperature influenced the inhibition of wheat germ agglutinin (WGA). Using enzyme-linked lectin assays (ELLAs), we observed biphasic inhibition profiles for all the dendrimers, characterized by an initial enhancement of WGA binding at low concentrations, followed by effective inhibition at higher concentrations.
View Article and Find Full Text PDFAdropin as a therapeutic candidate for HFpEF: evidence of oxidative stress mitigation via Nrf2/HO-1 signaling.
Lipids Health Dis
September 2025
Department of Cardiovascular Medicine, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, China.
Background: Heart failure with preserved ejection fraction (HFpEF), which accounts for more than half of all heart failure cases worldwide, has emerged as a major public health challenge characterized by substantial morbidity and mortality rates. As adropin is a key regulator of cardiovascular and metabolic homeostasis, this study investigated its therapeutic effects against HFpEF pathogenesis.
Methods: C57BL/6 mice were fed a high-fat diet (60% fat-derived calories) with NG-nitro-L-arginine methyl ester (L-NAME, 0.
Effect of particle size variation of stabilized wheat germ on dough processing characteristics and steamed bread quality.
Food Chem X
August 2025
Associated Discipline Key Laboratory of Whole Grain Nutrition and High-Value Utilization, Anhui Science and Technology University, 9 Donghua Road, Fengyang, 233100, China.
Wheat germ is a by-product of wheat flour milling and processing, and is valued for being the nutrient-rich center of wheat grains. In this study, hot air-stabilized wheat germ with five different particle sizes (coarse, medium, fine, ultrafine, and very fine) was prepared and mixed with wheat flour (WF) in a ratio of 8:92. The study focused on the effect of particle size of stabilized wheat germ on the thermomechanical, dynamic rheological, and fermentation properties of wheat flour and the quality of steamed bread.
View Article and Find Full Text PDFEx vivo liquid core fiber photometry with high-resolution 3D printing.
Sens Actuators Rep
December 2024
Department of Chemical and Environmental Engineering, University of California, Riverside, CA 92521, USA.
High resolution 3D printing emerges as an alternative to microfabrication due to its fine resolution along with one-step manufacturing. Thus, it is broadly used in many fields, such as biological and chemical applications. We introduce such a technique to the design of the optofluidic probe by integrating optics and microfluidics as an ex vivo liquid core fiber photometry.
View Article and Find Full Text PDF