New insights on the evolution of nucleolar dominance in newly resynthesized hexaploid wheat Triticum zhukovskyi.

Nucleolar dominance (ND) is a widespread epigenetic phenomenon in hybridizations where nucleolus transcription fails at the nucleolus organizer region (NOR). However, the dynamics of NORs during the formation of Triticum zhukovskyi (GGA A A A ), another evolutionary branch of allohexaploid wheat, remains poorly understood. Here, we elucidated genetic and epigenetic changes occurring at the NOR loci within the A , G, and D subgenomes during allopolyploidization by synthesizing hexaploid wheat GGA A A A and GGA A DD. In T. zhukovskyi, A genome NORs from T. timopheevii (GGA A ) were lost, while the second incoming NORs from T. monococcum (A A ) were retained. Analysis of the synthesized T. zhukovskyi revealed that rRNA genes from the A genome were silenced in F hybrids (GA A ) and remained inactive after genome doubling and subsequent self-pollinations. We observed increased DNA methylation accompanying the inactivation of NORs in the A genome and found that silencing of NORs in the S generation could be reversed by a cytidine methylase inhibitor. Our findings provide insights into the ND process during the evolutionary period of T. zhukovskyi and highlight that inactive rDNA units may serve as a 'first reserve' in the form of R-loops, contributing to the successful evolution of T. zhukovskyi.