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http://dx.doi.org/10.1093/jpids/piad023 | DOI Listing |
Mycoses
August 2025
Institute of Hygiene and Medical Microbiology, Medical University of Innsbruck, Innsbruck, Austria.
Background: Diagnosing invasive aspergillosis (IA) remains challenging despite the availability of various tests due to the limited sensitivity and variability in accuracy depending on the clinical context. Laboratory-based definitions consider different mycological criteria, such as culture and galactomannan (GM) positivity, equivalent in diagnostic weight. However, a more detailed analysis is essential for reliably distinguishing true infection from colonisation.
View Article and Find Full Text PDFClin Microbiol Infect
July 2025
Institute for Hygiene and Medical Microbiology, ECMM Excellence Diamond Centre, Medical University of Innsbruck, Innsbruck, Austria. Electronic address:
Background: Diagnosing invasive fungal infections (IFIs) is notoriously challenging. Test sensitivity and specificity vary with fungal burden, overlapping microscopic fungal appearances, lack of quantitative culture thresholds and, above all, the patient's immune status, specimen quality and prior antifungal exposure. These variables can mask or mimic disease, leading to delayed or erroneous treatment decisions.
View Article and Find Full Text PDFIran J Pathol
March 2025
Department of Infectious Diseases, Yas Hospital Complex, Tehran University of Medical Sciences, Tehran, Iran.
COVID-19-associated pulmonary aspergillosis (CAPA) is a complication of COVID-19. Galactomannan (GM) is a non-invasive test used to diagnose invasive aspergillosis. We collected the existing studies on the diagnostic value of GM to determine a GM level for predicting CAPA.
View Article and Find Full Text PDFInt J Mol Sci
April 2025
Institute for Theoretical and Applied Electromagnetics, Russian Academy of Sciences, 125412 Moscow, Russia.
Specific monosaccharide residue, β-D-galactofuranose (Galf) featuring a five-membered ring structure, is found in the glycans of fungi and bacteria, but is normally absent in healthy mammals and humans. In this study, synthetic oligosaccharides mimicking bacterial and fungal glycans were investigated by SERS (Surface-Enhanced Raman Scattering) techniques for the first time to distinguish between different types of glycan chains. SERS spectra of oligosaccharides related to fungal α-(1→2)-mannan, β-(1→3)-glucan, β-(1→6)-glucan, galactomannan of , galactan I of , and diheteroglycan of were measured.
View Article and Find Full Text PDFJ Fungi (Basel)
April 2025
Dipartimento di Scienze Biotecnologiche di Base, Cliniche Intensivologiche e Perioperatorie, Università Cattolica del Sacro Cuore, Largo A. Gemelli 8, 00168 Rome, Italy.
Invasive fungal diseases are a significant threat in immunocompromised patients, underscoring the need for rapid and accurate diagnostics. This study describes the development and validation of a real-time PCR-based laboratory-developed assay (LDA) on the Panther Fusion system for the simultaneous detection of (AF) and (PJ) in bronchoalveolar lavage fluid (BALF) samples. The assay was evaluated using 239 clinical BALF samples, including cases confirmed positive for AF or PJ by reference mycological methods.
View Article and Find Full Text PDF