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Article Abstract
Background: Tracking the emergence and spread of antimalarial drug resistance has become critical to sustaining progress towards the control and eventual elimination of malaria in South Asia, especially India.
Methods: An amplicon sequencing protocol was used for high-throughput molecular surveillance of antimalarial drug resistance in a total of 158 isolates at three sites in India: Chennai, Nadiad and Rourkela. Five genes of the implicated in antimalarial resistance were investigated here; for chloroquine resistance, for pyrimethamine resistance, for sulfadoxine resistance, for artemisinin resistance and for resistance to multiple antimalarials.
Results: Mutations in the propeller domain of PfK13 were observed in two samples only, however these mutations are not validated for artemisinin resistance. A high proportion of parasites from the dominant site Rourkela showed wild-type and haplotypes, while mutant and haplotypes were fixed at the dominant sites Chennai and Nadiad. The wild-type PfDHPS haplotype was predominant across all study sites. Finally, we observed the largest proportion of suspected multi-clonal infections at Rourkela, which has the highest transmission of among our study sites.
Conclusion: This is the first simultaneous high-throughput next generation sequencing of five complete genes from infected patients in India.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10104178 | PMC |
| http://dx.doi.org/10.1101/2023.04.08.23288321 | DOI Listing |
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