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Glyphosate has been the most widely used herbicide for decades providing a unique tool, alone or in mixtures, to control weeds on citrus in Veracruz. has developed glyphosate resistance for the first time in Mexico. The level and mechanisms of resistance of four resistant populations Rs (R1, R2, R3, and R4) were studied and compared with that of a susceptible population (). Resistance factor levels showed two moderately resistant populations (R2 and R3) and two highly resistant populations (R1 and R4). Glyphosate translocation through leaves to roots was ∼2.8 times higher in the S population than in the four R populations. A mutation (Pro106Ser) in the 2 gene was identified in the R1 and R4 populations. Mutation in the target site associated with reduced translocation is involved in increased glyphosate resistance in the R1 and R4 populations; whereas for the R2 and R3 populations, it was only mediated by reduced translocation. This is the first study of glyphosate resistance in from Mexico in which the resistance mechanisms involved are described in detail and control alternatives are proposed.
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http://dx.doi.org/10.1021/acs.jafc.2c07833 | DOI Listing |
Pest Manag Sci
September 2025
Saint-Jean-sur-Richelieu Research and Development Centre, Agriculture and Agri-Food Canada, Saint-Jean-sur-Richelieu, Quebec, Canada.
Background: Glyphosate resistance in Conyza canadensis (Canada fleabane) has been primarily attributed to non-target-site resistance (NTSR) mechanisms such as vacuolar sequestration, though these have not been formally elucidated. While a target-site mutation at EPSPS2 (P106S) was recently identified, it failed to account for many resistant cases. These findings underscore the need to re-evaluate the genetic basis of glyphosate resistance in this species.
View Article and Find Full Text PDFData Brief
October 2025
LMO Team, National Institute of Ecology, 1210 Geumgang-ro, Maseo-myeon, Seocheon 33657, Republic of Korea.
This dataset provides comprehensive profiles of bacterial and fungal communities associated with the holobionts of CP4-EPSPS-containing hybrids and wild-type in a natural roadside habitat. The hybrids were genetically consistent with × origin and possible backcrossing with , though the site and mechanism of hybridization are unclear. A total of 120 holobiont samples, including flowers, leaves, dead leaves, roots, and surrounding soil, were collected from twelve wild-type and twelve hybrid individuals (60 samples per group), in a natural roadside environment.
View Article and Find Full Text PDFIntern Emerg Med
August 2025
Clinica Medica "A. Murri", Department of Precision and Regenerative Medicine and Ionian Area (DiMePre-J), University of Bari "Aldo Moro" Medical School, Bari, Italy.
Environmental health should be an ethical obligation for experts in internal medicine. Besides the harmful health effects of air pollution, endocrine-disrupting chemicals and climate change, growing evidence points to glyphosate, a widely used herbicide, which generates risks to public health in terms of noncommunicable diseases and cancer. Detection of glyphosate in humans has been associated with all-cause mortality, increased frailty, insulin resistance, impaired glucose homeostasis and diabetes, increased risk of atherosclerotic cardiovascular diseases, fatty liver, and chronic obstructive pulmonary disease.
View Article and Find Full Text PDFPlant Sci
August 2025
IFEVA‑CONICET‑Department of Ecology, Faculty of Agronomy, University of Buenos Aires, Av. S. Martín 4453, Buenos Aires C1417DSE, Argentina. Electronic address:
Herbicide-resistant weeds are a prominent example of rapid global adaptation to new environments, significantly impacting the evolutionary ecology of plants, as well as the technology and economy of modern agriculture. In particular, the resistance of A. hybridus to glyphosate was found to be exceptionally high, and this has been recently attributed to a novel triple mutation in the EPSPS.
View Article and Find Full Text PDFPlants (Basel)
July 2025
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
"Rundao118" is a glyphosate-resistant rice; it contains both endogenous wild and mutated 5-enolpyruvylshikimate-3-phosphate synthase ( genes. Conventional qualitative and quantitative detection methods face significant challenges for direct analysis. Here, we describe five detection methods for identifying mutations in this rice line: (1) polymerase chain reaction (PCR) amplification-based Sanger sequencing, (2) next-generation sequencing (NGS) based on PCR amplification, (3) allele-specific PCR (AS-PCR), (4) real-time fluorescent quantitative PCR (qPCR), and (5) blocker displacement amplification (BDA).
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