sp. nov. sp. nov., sp. nov., sp. nov. and sp. nov., five novel lactic acid bacteria isolated from insects or flowers of Kangaroo Island, South Australia.

Six strains, KI11_D11, KI4_B1, KI11_C11, KI16_H9, KI4_A6 and KI3_B9, were isolated from insects and flowers on Kangaroo Island, South Australia. On the basis of 16S rRNA gene phylogeny, strains KI11_D11, KI4_B1, KI11_C11, KI16_H9, KI4_A6 were found to be closely related to Ru20-1. Due to the lack of a whole genome sequence for this species, whole genome sequencing of Ru20-1 was undertaken. KI3_B9 was found to be closely related to F214-1. Utilizing core gene phylogenetics and whole genome analyses, such as determination of AAI, ANI and dDDH, we propose that these six isolates represent five novel species with the names (KI11_D11= LMG 32130 = NBRC 114988), (KI11_C11 = LMG 32129 = NBRC 114987), (KI16_H9= LMG 32131 = NBRC 114989) (KI4_A6 = LMG 32127 = NBRC 114985) and (KI3_B9 = LMG 32124 = NBRC 114983). Chemotaxonomic analyses detected no fructophilic characters for these strains of member of the genus . KI3_B9 was found to be obligately fructophilic, similarly to its phylogenetic neighbours in the genus . This study represents the first isolation, to our knowledge, of novel species in the family from the Australian wild.