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Despite the advantages of the Anaerobic Digestion (AD) technology for organic waste management, low system performance in biogas production negatively affects the wide spread of this technology. This paper develops a new artificial intelligence-based framework to predict and optimise the biogas generated from a micro-AD plant. The framework comprises some main steps including data collection and imputation, recurrent neural network/ Non-Linear Autoregressive Exogenous (NARX) model, shuffled frog leaping algorithm (SFLA) optimisation model and sensitivity analysis. The suggested framework was demonstrated by its application on a real micro-AD plant in London. The NARX model was developed for predicting yielded biogas based on the feeding data over preceding days in which their lag times were fine-tuned using the SFLA. The optimal daily feeding pattern to obtain maximum biogas generation was determined using the SFLA. The results show that the developed framework can improve the productivity of biogas in optimal operation strategy by 43 % compared to business as usual and the average biogas produced can raise from 3.26 to 4.34 m/day. The optimal feeding pattern during a four-day cycle is to feed over the last two days and thereby reducing the operational costs related to the labour for feeding the plant in the first two days. The results of the sensitivity analysis show the optimised biogas generation is strongly influenced by the content of oats and catering waste as well as the optimal allocated day for adding feed to the main digester compared to other feed variables e.g., added water and soaked liner.
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http://dx.doi.org/10.1016/j.wasman.2022.12.034 | DOI Listing |
Antonie Van Leeuwenhoek
September 2025
Microbiology Area, Bioscience Department, Facultad de Química, Universidad de la República (UdelaR), Gral. Flores 2124, Montevideo, Uruguay.
The global oversupply of crude glycerol, a byproduct of biodiesel production, needs innovative strategies for its sustainable utilization. In this study we isolated and characterized oleaginous yeast strains from fruit surfaces in the Brazilian Cerrado biome, a biodiversity hotspot, to assess their potential for converting crude glycerol into microbial lipids suitable for biodiesel. From 150 fruits, 45 yeast strains were isolated, with six identified as oleaginous (intracellular lipids > 20% dry biomass).
View Article and Find Full Text PDFNat Commun
August 2025
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China.
Non-canonical amino acids (ncAAs) bearing diverse functional groups hold transformative potential in drug discovery, protein engineering, and biomaterial science. However, their industrial-scale production remains constrained by the inefficiency, high cost, and environmental burden of conventional chemical and enzymatic methods. Here, we present a modular multi-enzyme cascade platform that leverages glycerol-an abundant and sustainable byproduct of biodiesel production-as a low-cost substrate for ncAAs synthesis.
View Article and Find Full Text PDFBioresour Technol
August 2025
Material Cycles Division, National Institute for Environmental Studies, Tsukuba 305-8506, Japan. Electronic address:
Anaerobic digestion is a promising waste-to-energy method for treating organic solid waste. However, the unavoidable generation of CO decreased its calorific value and potential application. This study presented a novel method to enhance the methane yield and decrease CO production of digestion by coupling biochar and hydrogen.
View Article and Find Full Text PDFBioengineering (Basel)
July 2025
Swedish Centre for Resource Recovery, University of Borås, 50190 Borås, Sweden.
Anaerobic digestion (AD) has long been valued for producing a biogas-digestate pair, yet its profitability is tightening. Next-generation AD biorefineries now position syngas both as a supplementary feedstock and as a springboard to capture high-value intermediates, hydrogen (H) and volatile fatty acids (VFA). This review dissects how complex natural consortia "decide" between hydrogenogenesis and acetogenesis when CO, H, and CO co-exist in the feedstocks, bridging molecular mechanisms with process-scale levers.
View Article and Find Full Text PDFEnviron Technol
August 2025
Department of Sanitary and Environmental Engineering, Federal University of Minas Gerais, Belo Horizonte, Brazil.
Decentralized anaerobic treatment presents a sustainable alternative for managing the substantial generation of food waste. However, the associated high costs may render this option less feasible for small- to medium-scale systems. To explore a novel strategy for cost reduction in anaerobic digestion systems used for food waste treatment, this study applied organic loading rates (OLR) ranged from 0.
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