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The Usutu virus (USUV) has recently attracted the attention of scientists because of its rapid spread across Europe and its growth over the previous seasons in Italy. Here, we describe the first case of USUV infection in Asti, Piedmont region, Italy. The patient remained asymptomatic in the acute phase and during the early follow-up, despite a mild increase in liver enzymes. The prompt diagnosis in this patient was due to positive qualitative PCR for WNV blood-donor screening with negative RT-PCR of WNV and positive USUV-RNA following the confirmation test. Blood-donor screening and transmission risk monitoring are pivotal in following the spread of this Flavivirus in non-endemic countries, due to the high percentage of asymptomatic carriers.
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http://dx.doi.org/10.3390/tropicalmed7120443 | DOI Listing |
PLoS Negl Trop Dis
September 2025
Pathogenesis and Control of Chronic and Emerging Infections (PCCEI), Université de Montpellier, Inserm, Montpellier, France.
The Usutu virus, a neurotropic Orthoflavivirus transmitted by mosquitoes, was first identified in South Africa in 1959 and has progressively spread across Europe over the past two decades. This virus follows an enzootic cycle between mosquitoes and birds, leading to periodic outbreaks that have caused significant bird mortality. Although primarily an avian pathogen, Usutu virus can occasionally infect humans and other mammals who act as incidental or dead-end hosts.
View Article and Find Full Text PDFNat Commun
August 2025
Viroscience, Erasmus MC, Rotterdam, the Netherlands.
Mosquito-borne arboviruses, including Usutu virus (USUV) and West Nile virus (WNV), are emerging threats in Europe, with changes in climate, land use shifts, and increasing global connectivity influencing their dynamics. Understanding how these viruses emerge and establish in new regions is critical for mitigating risks and improving public and wildlife health preparedness. Here, we present a seven-year study (2016-2022, inclusive) documenting the emergence and spread of USUV and WNV in the Netherlands.
View Article and Find Full Text PDFFront Vet Sci
July 2025
Veterinary Faculty, University of Sarajevo, Sarajevo, Bosnia and Herzegovina.
Introduction: With their remarkable flight capabilities, wild and captive birds play a pivotal role in the global dissemination of zoonotic pathogens including , Avian Influenza viruses (AIV), Chikungunya virus (CHIKV), Usutu virus (USUV), and West Nile virus (WNV). They function both as hosts and reservoirs responsible for transporting the mentioned infectious agents across vast geographic regions. Additionally, captive birds and birds inhabiting urban environments, particularly in tourist destinations, present significant public health concerns due to facilitated close interactions with humans.
View Article and Find Full Text PDFSci Rep
August 2025
Fraunhofer Institute for Cell Therapy and Immunology, Department of Infection Research and Diagnostics, Perlickstraße 1, 04103, Leipzig, Germany.
West Nile virus (WNV), Usutu virus (USUV) and tick-borne encephalitis virus (TBEV) are worldwide endemic zoonotic orthoflaviviruses, often co-circulating in the same areas. Serological studies in animals, mostly birds and horses, are important means to monitor the spread of these viruses and the infection risks for humans. However, cross-reactive antibodies to these structurally similar flaviviruses frequently impact serological differentiation in enzyme-linked immunosorbent assays (ELISAs), hence time-consuming virus neutralization tests (VNTs) have to be employed in laboratories with high biosafety level.
View Article and Find Full Text PDFParasit Vectors
August 2025
Institute of Infection, Veterinary and Ecological Sciences, Faculty of Health and Life Sciences, University of Liverpool, Liverpool, L69 3BX, UK.
Background: With medically important arboviruses such as West Nile virus (WNV) circulating in Europe and Usutu virus (USUV) currently present in the UK, it is imperative to identify areas in the UK at risk of establishment and spread of these viruses. Here, we describe a comprehensive nationwide field surveillance study conducted during July 2023 to map the distribution of the WNV and USUV competent vectors: Culex pipiens biotype pipiens, Culex pipiens biotype molestus and Culex torrentium, across England and Wales.
Methods: Mosquitoes were sampled for 3 trap nights (3TN) at 200 sites in rural, urban and suburban settings, selected using a lattice plus close pairs surveillance design.