Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Here we describe the design and the characterization of novel electrode materials consisting of multi-walled carbon nanotubes coated with glyconanoparticles (GNPs) functionalized with anthraquinone sulfonate. The resulting modified electrodes were characterized by scanning electron microscopy and cyclic voltammetry. Their electrochemical behavior reveals a stable pH-dependent redox signal characteristic of anthraquinone sulfonate. Immobilization of bilirubin oxidase on these three-dimensional electrodes leads to the electroenzymatic reduction of O to water with an onset potential of 0.5 V/SCE (saturated calomel electrode). A catalytic cathodic current of 174 µA (0.88 mA cm) at 0.1 V/SCE, demonstrates that glyconanoparticles modified by anthraquinone sulfonate were able to interact and orientate bilirubin oxidase by electrostatic interactions.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.bioelechem.2022.108328 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Magnetic field-enhanced transformation of biochemical energy into motion of enzyme-modified graphene monolayers.
Chem Commun (Camb)
August 2025
Engineering Research Center for Nanomaterials, Henan University, 475000, Kaifeng, China.
This contribution studies enzymatically driven graphene monolayer swimmers for the direct transformation of biochemical energy into motion. The swimmers are elaborated bipolar electrodeposition of gold and asymmetric immobilization of glucose oxidase and bilirubin oxidase on small pieces of a graphene monolayer. Most importantly, we demonstrate that the speed of the free-standing hybrid objects can be enhanced by an external magnetic field, allowing also controlled rotation, despite the absence of ferromagnetic construction elements.
View Article and Find Full Text PDFPhotosystem II-Carbon Nitride Photoanodes for Scalable Biophotoelectrochemistry.
Adv Mater
August 2025
School of Chemical Engineering, The University of Adelaide, Adelaide, SA 5005, Australia.
Photosystem II (PSII) is a vital photosynthetic enzyme with the potential for sustainable bioelectricity and fuel generation. However, interfacing PSII with intricate, small-scale electrodes for practical applications has been challenging. This study addresses this by creating protonated macroporous carbon nitride (MCN) as support and developing a scalable spray-freeze method to wire PSII with MCN.
View Article and Find Full Text PDFGenetic Surfaceome E. coli Reprogramming Enables Selective Water Oxidation.
Adv Mater
August 2025
Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
Programming catalytic behavior at the microbial genome level is a frontier in synthetic biology with direct impact on bioelectrocatalysis. A key challenge is the coordinated control of gene expression, localization, folding, and cofactor maturation required to achieve proper bioelectrocatalytic activity. Here, a synthetic operon in Escherichia coli is engineered to reprogram its surfaceome for selective water oxidation.
View Article and Find Full Text PDFConfocal Absorbance-Activated Droplet Sorting (cAADS) for Enzyme Engineering.
Adv Sci (Weinh)
August 2025
Centre de Recherche Paul Pascal (CRPP), CNRS UMR 5031, Univ. Bordeaux, 115 Avenue du Docteur Schweitzer, Pessac, 33600, France.
Directed evolution relies on iterative cycles of variant generation, screening, and selection to identify enzyme variants with improved activities. Droplet-based microfluidics accelerates this process by enabling rapid screening of enzyme variants in water-in-oil emulsions acting as picoliter-scale microcompartments. In fluorescence-activated droplet sorting (FADS), single E.
View Article and Find Full Text PDFSelf-powered detection of T4 polynucleotide kinase activity based on DNA structure transformation-modulated direct electron transfer of bilirubin oxidase.
Talanta
August 2025
Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education of China), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, 410081, China. Electronic address:
Enzymatic biofuel cell-based self-powered sensors represent a promising class of portable sensing devices, so the development of a novel and efficient self-powered sensing strategy is of critical importance. Herein, the direct electron transfer (DET) of bilirubin oxidase (BOD) is modulated through enzymatic reaction-triggered DNA structure transformation, which is further applied for self-powered detection of T4 polynucleotide kinase (T4 PNK) activity. The biocathode of a glucose/oxygen biofuel cell is prepared by immobilizing BOD on carbon nanotubes (MWCNTs)-gold nanoparticles (AuNPs) nanocomposite by using a short-stranded DNA (sDNA)-complementary DNA (cDNA) duplex as a bridge.
View Article and Find Full Text PDF