Comprehensive Analysis of Gene Family and Its Expression in Response to Different Abiotic Stresses in Potato.

As a highly conserved family of plant-specific proteins, SIMILAR-TO-RCD-ONE (SROs) play an essential role in plant growth, development and response to abiotic stresses. In this study, six genes were identified by searching the PARP, RST and WWE domains based on the genome-wide data of potato database DM v6.1, and they were named - according to their locations on chromosomes. genes were comprehensively analyzed using bioinformatics methods. The results showed that six genes were irregularly distributed on five chromosomes. Phylogenetic analysis showed that 30 genes of four species were distributed in three groups, while genes were distributed in groups II and III. The promoter sequence of genes contained many cis-acting elements related to hormones and stress responses. In addition, the expression level of genes in different tissues of doubled monoploid (DM) potato, as well as under salt, drought stresses and hormone treatments, was analyzed by RNA-seq data from the online database and quantitative real-time polymerase chain reaction (qRT-PCR) analysis. Furthermore, the expression level of genes was analyzed by transcriptome analysis under mild, moderate and severe salt stress. It was concluded that genes could respond to different abiotic conditions, but their expression level was significantly different. This study lays a foundation for further studies on the biological functions of the gene family.