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For monitoring of the residual of parathion pesticide in food, herein, a sensitive and reliable electrochemical immunosensor based on cross-linked polyvinyl alcohol/citric acid nanofiber membrane (PVA/CA NFM) and horseradish peroxidase labeled anti-parathion nanobody was constructed. Firstly, the cross-linked PVA/CA NFM with extra-high surface area and uniform morphology was prepared and characterized. Then, the immunosensor was assembled and its analytical performances were evaluated. It exhibited high specificity and sensitivity to parathion with the liner range and limit of detection being 0.01-100 ng/mL and 2.26 pg/mL, respectively. Moreover, the biosensor kept almost 75% of its initial activity after regenerating 4 times, and remained 85% after 9 weeks of storage. Finally, the average recoveries from food samples were 96.20%-114.61% with the coefficient of variation being 1.06%-5.28%, which was correlate well with UPLC (R = 0.9964). Therefore, the sensor was demonstrated to be a feasible alternative for sensitive assay of parathion.
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http://dx.doi.org/10.1016/j.foodchem.2022.134371 | DOI Listing |
Bioelectrochemistry
August 2025
Department of General Surgery, Hui Ya Hospital of The First Affiliated Hospital, Sun Yat-Sen University, Huizhou, Guangdong 516081, China. Electronic address:
Human epidermal growth factor receptor-2 (HER-2), a key biomarker in breast cancer, is critical for early diagnosis and prognosis evaluation. In this work, a label-free electrochemical immunosensor was developed for biomarker HER-2 detection based on PtSnCoNi hierarchical dendritic alloyed nanowires (PtSnCoNi HDNWs). These nanowires were synthesized via a co-reduction-triggered anisotropic growth strategy in oleylamine.
View Article and Find Full Text PDFAnal Chim Acta
November 2025
The Key Lab of Health Chemistry & Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering & Materials Science, Soochow University, Suzhou, 215123, PR China; Center of Self-Propelled Nanotechnologies, Suzhou Industrial Park Institute of Services Outsourcing, Suzhou, 215123, PR China
Background: Of the mycotoxins, aflatoxin is the most significant. The detection of aflatoxin B1 (AFB1) is crucial for ensuring food safety, as this highly carcinogenic toxin readily contaminates crops such as grains and nuts, and timely detection can effectively prevent associated health risks. The selection of luminophores is of paramount importance in the detection of ECL (electrochemiluminescence).
View Article and Find Full Text PDFMikrochim Acta
September 2025
College of Medical Technology, Shaanxi University of Chinese Medicine, Xianyang, 712000, Shaanxi, China.
An advanced electrochemical immunosensor platform was designed for the precise quantification of cortisol. The sensor design integrates graphene oxide-silicon carbide (GO-SiC) nanocomposites onto a glassy carbon electrode (GCE). Denatured bovine serum albumin (d-BSA) and an anti-cortisol antibody were immobilized on the GO-SiC/GCE surface as part of the immunosensor's design.
View Article and Find Full Text PDFBioelectrochemistry
August 2025
Department of Physical Chemistry, Institute of Chemistry, Faculty of Chemistry and Geosciences, Vilnius University, Naugarduko St. 24, LT-03225 Vilnius, Lithuania; Department of Nanotechnology, State Research Institute Center for Physical and Technological Sciences, Sauletekio Ave. 3, LT-10257 Vilni
The emergence of SARS-CoV-2 has posed significant global health challenges. The nucleocapsid protein (N-protein) is a structural part of the SARS-CoV-2 virus and an important immunogenic target of specific antibodies, which are developed in the organism during the infection by this virus. Artificially designed specific (monoclonal and polyclonal) antibodies are also used for therapeutic and bioanalytical purposes, therefore, the assessment and characterization of newly designed antibodies is an important analytical issue.
View Article and Find Full Text PDFMikrochim Acta
September 2025
The Second People's Hospital of Qingyang City, Qingyang City, Gansu, 745000, China.
An electrochemical immunosensor was successfully developed for evaluating traditional Chinese medicine (TCM)-induced cardiac toxicity by immobilizing heart-type fatty acid-binding protein (HFABP)-specific antibodies on an electrode surface modified with chitosan (CS)-functionalized metal-organic framework ZIF-8 and gold nanoparticles (AuNPs). Leveraging the excellent biocompatibility of AuNPs, the excellent stability and high surface area of ZIF-8, along with the strong specific recognition capability of HFABP antibodies, the sensor demonstrated superior performance. Under optimized experimental conditions, it achieved an ultra-low detection limit of 7.
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