Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
The genomic profiling of circulating tumor cells (CTCs) in the bloodstream should provide clinically relevant information on therapeutic efficacy and help predict cancer survival. Here, we contrasted the genomic profiles of CTC pools recovered from metastatic colorectal cancer (mCRC) patients using different enrichment strategies (CellSearch, Parsortix, and FACS). Mutations inferred in the CTC pools differed depending on the enrichment strategy and, in all cases, represented a subset of the mutations detected in the matched primary tumor samples. However, the CTC pools from Parsortix, and in part, CellSearch, showed diversity estimates, mutational signatures, and drug-suitability scores remarkably close to those found in matching primary tumor samples. In addition, FACS CTC pools were enriched in apparent sequencing artifacts, leading to much higher genomic diversity estimates. Our results highlight the utility of CTCs to assess the genomic heterogeneity of individual tumors and help clinicians prioritize drugs in mCRC.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.ygeno.2022.110500 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
DGARM/C10orf76/ARMH3 for Ceramide Transfer Zone at the Endoplasmic Reticulum-Distal Golgi Contacts.
Contact (Thousand Oaks)
March 2024
Department of Quality Assurance, Radiation Safety and Information System, National Institute of Infectious Diseases, Tokyo, Japan.
Phosphatidylinositol 4-monophosphate (PtdIns(4)P) is one of the key membrane components which mark the membrane contact sites. In the mammalian Golgi complex, PtdIns(4)P is produced at various subregions via specific mechanisms for each site. Particularly, PtdIns(4)P pools generated at the distal Golgi regions are pivotal for the determination of membrane contacts between the endoplasmic reticulum (ER) and Golgi, at which inter-organelle lipid transport takes place.
View Article and Find Full Text PDFUpregulated hepatic lipogenesis from dietary sugars in response to low palmitate feeding supplies brain palmitate.
Nat Commun
January 2024
Department of Nutritional Sciences, University of Toronto, 1 King's College Circle, Toronto, M5S 1A8, ON, Canada.
Palmitic acid (PAM) can be provided in the diet or synthesized via de novo lipogenesis (DNL), primarily, from glucose. Preclinical work on the origin of brain PAM during development is scarce and contrasts results in adults. In this work, we use naturally occurring carbon isotope ratios (C/C; δC) to uncover the origin of brain PAM at postnatal days 0, 10, 21 and 35, and RNA sequencing to identify the pathways involved in maintaining brain PAM, at day 35, in mice fed diets with low, medium, and high PAM from birth.
View Article and Find Full Text PDFHumans as blood-feeding sources in sylvatic triatomines of Chile unveiled by next-generation sequencing.
Parasit Vectors
July 2023
Departamento de Ciencias Ecológicas, Facultad de Ciencias, Universidad de Chile, Santiago, Chile.
Background: Triatomines are blood-sucking insects capable of transmitting Trypanosoma cruzi, the parasite that causes Chagas disease in humans. Vectorial transmission entails an infected triatomine feeding on a vertebrate host, release of triatomine infective dejections, and host infection by the entry of parasites through mucous membranes, skin abrasions, or the biting site; therefore, transmission to humans is related to the triatomine-human contact. In this cross-sectional study, we evaluated whether humans were detected in the diet of three sylvatic triatomine species (Mepraia parapatrica, Mepraia spinolai, and Triatoma infestans) present in the semiarid-Mediterranean ecosystem of Chile.
View Article and Find Full Text PDFComparative analysis of capture methods for genomic profiling of circulating tumor cells in colorectal cancer.
Genomics
November 2022
CINBIO, Universidade de Vigo, 36310 Vigo, Spain; Galicia Sur Health Research Institute (IIS Galicia Sur), SERGAS-UVIGO, Spain; Department of Biochemistry, Genetics, and Immunology, Universidade de Vigo, 36310 Vigo, Spain. Electronic address:
Article Synopsis
- The study investigates how analyzing circulating tumor cells (CTCs) from blood can provide valuable insights into cancer treatment effectiveness and survival predictions for metastatic colorectal cancer (mCRC) patients.
- Researchers compared different methods for collecting CTCs—CellSearch, Parsortix, and FACS—and found that the genomic mutations varied based on the method used.
- Results showed that CTCs from Parsortix and CellSearch methods presented genomic features similar to those of the primary tumors, while FACS enrichment resulted in misleading diversity estimates due to sequencing errors; this underscores the potential of CTC analysis in personalizing cancer treatment.
Implementation of an Integrated Dielectrophoretic and Magnetophoretic Microfluidic Chip for CTC Isolation.
Biosensors (Basel)
September 2022
Liaoning Key Laboratory of Marine Sensing and Intelligent Detection, Dalian Maritime University, Dalian 116026, China.
Identification of circulating tumor cells (CTCs) from a majority of various cell pools has been an appealing topic for diagnostic purposes. This study numerically demonstrates the isolation of CTCs from blood cells by the combination of dielectrophoresis and magnetophoresis in a microfluidic chip. Taking advantage of the label-free property, the separation of red blood cells, platelets, T cells, HT-29, and MDA-231 was conducted in the microchannel.
View Article and Find Full Text PDF