Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Retinoblastoma (RB) is the most common paediatric intraocular tumour. The management of RB has improved the survival and vision with recent advances in the treatment. Improved therapeutic approaches focussing on targeting tumours and minimizing the treatment-associated side effects are being developed. In this study, we generated a ssDNA aptamer against RB by cell-SELEX and high-throughput sequencing using Weri-RB1 cell line as the target, and Muller glial cell line Mio-M1 as the control. Three aptamers were selected based on the number of repetitions in NGS and phylogenetic relationship and evaluated by flow cytometry to assess their binding affinity and selectivity. The dissociation constant, Kd values of three selected aptamers were found to be in the nanomolar range. Aptamer VRF-CSRB-01 with the best binding affinity and a Kd value of 49.41 ± 7.87 nM was further characterized. The proteinase and temperature treatment indicated that VRF-CSRB-01 targets surface proteins, and has a good binding affinity and excellent selectivity under physiological conditions. The aptamer VRF-CSRB-01 was stable over 72 h in serum and 96 h in cerebral spinal fluid and vitreous. With the high affinity, specificity, stability and specific recognition of clinical RB tumours, VRF-CSRB-01 aptamer holds potential for application in diagnosis and targeting RB.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9519959 | PMC |
| http://dx.doi.org/10.1038/s41598-022-20660-3 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Antiemetic drug fosaprepitant exerts anti-tumor effects against NSCLC by targeting FAK to inhibit AKT and JNK/c-Jun pathways.
Acta Pharmacol Sin
September 2025
Department of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of Soochow University, Suzhou 215006, China.
Non-small cell lung cancer (NSCLC) is an aggressive malignancy with a poor prognosis. Abnormal expression of focal adhesion kinase (FAK) is closely linked to NSCLC progression, highlighting the need for effective FAK inhibitors in NSCLC treatment. In this study we conducted high-throughput virtual screening combined with cellular assays to identify potential FAK inhibitors for NSCLC treatment.
View Article and Find Full Text PDFTargeted hotspot profiling reveals a functionally relevant mutation in bladder cancer.
Urol Oncol
September 2025
Nutritional, Genes and Human Disease Laboratory, Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka, Bangladesh. Electronic address:
Background: Understanding the mutational landscape is critical for elucidating the molecular mechanisms driving cancer progression. This study aimed to profile somatic mutations in bladder cancer patients (N=7) from Bangladesh to provide insights into the genetic alterations underlying this malignancy.
Methods: We performed targeted sequencing of 50 oncogenes and tumor suppressor genes using the Ion AmpliSeq Cancer Hotspot Panel v2 on tumor and matched blood samples from seven bladder cancer patients.
Molecular basis for the recognition of low-frequency polyadenylation signals by mPSF.
Nucleic Acids Res
September 2025
Department of Biological Sciences, Columbia University, New York, NY 10027, United States.
The 3'-end cleavage and polyadenylation of pre-mRNAs is dependent on a key hexanucleotide motif known as the polyadenylation signal (PAS). The PAS hexamer is recognized by the mammalian polyadenylation specificity factor (mPSF). AAUAAA is the most frequent PAS hexamer and together with AUUAAA, the second most frequent hexamer, account for ∼75% of the poly(A) signals.
View Article and Find Full Text PDFGalectin-1-conjugated gold nanoparticles as a multivalent macromolecular platform for broad-spectrum inhibition of influenza virus via glycan recognition.
Int J Biol Macromol
September 2025
School of Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan. Electronic address:
The development of antiviral nanotherapeutics remains a formidable challenge due to the structural diversity and rapid evolution of viral surface glycoconjugates. Here, we report a rationally engineered multivalent Galectin-1 (Gal-1) nanoplatform based on 13-nm gold nanoparticles (AuNPs) for high-affinity glycan targeting and therapeutic inhibition of influenza virus. By leveraging site-specific conjugation and molecular orientation control, the AuNP/Gal-1 nanocomplex maximizes the exposure of carbohydrate recognition domains (CRDs) while preserving Gal-1's tertiary structure, as confirmed by molecular dynamics simulations and spectroscopic analyses.
View Article and Find Full Text PDFPotential Anticoagulant Mechanism of Curdione: Enhancing Thrombin Inhibition by Binding to AT-Ⅲ 's D-Helix Region.
J Ethnopharmacol
September 2025
Department of Pharmacy, The First Affiliated Hospital of Anhui Medical University, Hefei, China. Electronic address:
Ethnopharmacological Relevance: Curcuma wenyujin was first recorded in the Tang Dynasty's Xinxiu Bencao and has been traditionally used to treat blood stasis syndrome. Its active component curdione exhibits antiplatelet effects, though its anticoagulant mechanisms remain unclear and require further investigation.
Aim Of The Study: To investigate the anticoagulant activity of curdione, identify potential targets through integrated screening, and elucidate the underlying mechanisms.