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A novel lytic siphophage, PLG-II, which is specific for Lactococcus garvieae serotype II strains that are pathogenic to fish, was isolated from seawater samples collected from Miyazaki Prefecture, Japan. Whole-genome sequencing showed that the PLG-II genome is a 32,271-bp double-stranded DNA molecule, with an average GC content of 37.74%. It contains 69 open reading frames (ORFs), 43 of which currently have no reliable functional annotation for their product, as well as a single tRNA. Comparative genomics analysis suggests that phage PLG-II might represent a novel species in the genus Uwajimavirus.
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http://dx.doi.org/10.1007/s00705-022-05568-7 | DOI Listing |
ACS Infect Dis
September 2025
Animal-Derived Food Safety Innovation Team, College of Veterinary Medicine, Anhui Agricultural University, Hefei 230036, China.
The emergence of multidrug-resistant (MDR) poses a significant threat to global public health, necessitating alternative therapeutic strategies. In this study, we isolated and characterized a novel lytic bacteriophage (phage), vB_EcoM_51, from poultry farm sewage and evaluated its potential against MDR . Transmission electron microscopy revealed that the phage exhibits morphological features typical of the family, including a polyhedral head (∼66.
View Article and Find Full Text PDFAPMIS
September 2025
Department of Molecular Biology and Genetics, Tokat Gaziosmanpasa University, Tokat, Türkiye.
Pyroptosis is a lytic and pro-inflammatory regulated cell death pathway mediated by pores formed by the oligomerization of gasdermin proteins on cellular membranes. Different pro-inflammatory molecules such as interleukin-18 are released from these pores, promoting inflammation. Pyroptotic cell death has been implicated in many pathological conditions, including cancer and liver diseases.
View Article and Find Full Text PDFVirology
September 2025
Department of Microbiology, Faculty of Pharmacy, Ahram Canadian University (ACU), 6th October City, Giza, 12566, Egypt. Electronic address:
Background: Carbapenem-resistant Acinetobacter baumannii (CRAB) has recently become a serious cause for global concern because of non-susceptibility to multiple antimicrobial classes, its prevalence in nosocomial infections, and the lack of effective treatments against such a pathogen.
Methods: This study isolated two lytic phages from hospital sewage, purified, propagated, characterized morphologically by transmission electron microscopy, and genomically by Oxford Nanopore Sequencing. The phage lysates were then formulated individually as carboxymethylcellulose (CMC) 5 % w/v hydrogels.
Microb Pathog
September 2025
College of Life Sciences and Technology, Beijing University of Chemical Technology, Beijing, 100029, China; Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology, Beijing, 100029, China. Electronic address:
The antibiotic-resistant Enterococcus faecium (E. faecium) is a significant health issue requiring alternative therapies. Phages could be an alternative to antibiotics and have promising activity in both in vitro and in vivo experiments.
View Article and Find Full Text PDFVet J
September 2025
Division of Biological Science, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand; Center of Antimicrobial Biomaterial Innovation-Southeast Asia, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand. Electronic address:
Multidrug-resistant avian pathogenic Escherichia coli (MDR-APEC) causes high mortality in newborn chickens, leading to significant economic losses for poultry industry worldwide. The present study proposes an effective alternative strategy to control APEC infections by using bacteriophage specific to MDR-APEC. Lytic phage CABI-SEA 2301 isolated from a chicken faecal sample collected at a commercial poultry farm using the double layer agar overlay technique was classified as a novel species in the genus Seuratvirus, subfamily Queuovirinae.
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