Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Current strategies for fertility preservation include the cryopreservation of embryos, mature oocytes or ovarian cortical tissue for autologous transplantation. However, not all patients that could benefit from fertility preservation can use the currently available technology. In this regard, obtaining functional mature oocytes from ovarian cortical tissue would represent a major breakthrough in fertility preservation as well as in human medically assisted reproduction. In this study, we have used a microfluidics platform to culture cryopreserved-thawed human cortical tissue for a period of 8 days and evaluated the effect of two different flow rates in follicular activation and growth. The results showed that this dynamic system supported follicular development up to the secondary stage within 8 days, albeit with low efficiency. Surprisingly, the stromal cells in the ovarian cortical tissue were highly sensitive to flow and showed high levels of apoptosis when cultured under high flow rate. Moreover, after 8 days in culture, the stromal compartment showed increase levels of collagen deposition, in particular in static culture. Although microfluidics dynamic platforms have great potential to simulate tissue-level physiology, this system still needs optimization to meet the requirements for an efficient early follicular growth.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9372461 | PMC |
| http://dx.doi.org/10.3389/fendo.2022.936765 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Case Report: Acute polymorphic psychosis and NMDA-R IgG antibodies in serum: a follow-up case study.
Front Immunol
September 2025
Department of Psychiatry and Psychotherapy, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany.
Introduction: Anti-N-methyl-D-aspartate receptor (NMDA-R) encephalitis is a neuropsychiatric disorder with additional psychiatric features caused by NMDA-R immunoglobulin G (IgG) antibodies in cerebrospinal fluid (CSF). This report presents the follow-up of a patient in whom we assumed mild NMDA-R encephalitis in the first psychotic episode.
Case Study: A patient with a prior episode of an acute polymorphic psychotic syndrome relapsed five and a half years later following a severe COVID-19 infection.
Integrating Tissue and Cytoplasmic Rigidity Transitions During Morphogenesis.
Dev Growth Differ
September 2025
Laboratory for Epithelial Morphogenesis, RIKEN Center for Biosystems Dynamics Research, Kobe, Japan.
Multicellular organisms generate organizational complexity through morphogenesis, in which mechanical forces orchestrate the movements and deformations of cells and tissues, while chemical signals regulate the molecular events that generate and coordinate these forces. One common denominator that is critical both for mechanics and biochemistry is material property. Material properties define how materials deform or rearrange under applied forces, and how rapidly molecules interact or spread in space and time.
View Article and Find Full Text PDFProtection of the Endothelium and Endothelial Glycocalyx by Albumin and Sulodexide in Porcine Model of Kidney Transplant.
Exp Clin Transplant
August 2025
>From the Department of Urology, University Hospital Hradec Kralove, Hradec Kralove, Czechia; and the Charles University, Faculty of Medicine in Hradec Kralove, Hradec Kralove, Czechia.
Objectives: Kidney transplant is a life-saving procedure for patients with end-stage renal disease. Success of kidney transplant is highly dependent on maintaining the integrity of the endothelium and its protective layer, the endothelial glycocalyx. Ischemia-reperfusion injury, a common challenge in kidney transplant, can disrupt the endothelial glycocalyx, leading to various post-transplant complications.
View Article and Find Full Text PDFStudy of a near-cortical over-drilling technique on plate constructs with a conical locking system in a rabbit femoral fracture using a finite element model.
Med Eng Phys
October 2025
Centre for Simulation in Bioengineering, Biomechanics and Biomaterials (CS3B), Department of Mechanical Engineering, School of Engineering of Bauru, São Paulo State University (UNESP), Bauru, São Paulo, Brazil. Electronic address:
This study aimed to evaluate the near-cortical over-drilling technique on the mechanical behaviour of bone-plate constructs in a rabbit transverse femoral fracture. In vitro biomechanical testing and finite element (FE) models were used for analyses. Rabbits' bones (n = 14) were divided into two groups: G1 - without near-cortical over-drilling, and G2 - with near-cortical over-drilling.
View Article and Find Full Text PDFA clinical and genotype-phenotype analysis of MACF1 variants.
Am J Hum Genet
September 2025
Department of Clinical Genetics, Erasmus MC, University Medical Center Rotterdam, PO Box 2040, Rotterdam 3000 CA, the Netherlands.
Microtubule-actin cross-linking factor 1 (MACF1) is a large protein of the spectraplakin family, which is essential for brain development. MACF1 interacts with microtubules through the growth arrest-specific 2 (Gas2)-related (GAR) domain. Heterozygous MACF1 missense variants affecting the zinc-binding residues in this domain result in a distinctive cortical and brain stem malformation.
View Article and Find Full Text PDF