Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
A simple and sensitive Mn-ZnS quantum dot room-temperature phosphorescent immunosensor for detecting microcystin-LR was developed. This sensor adopted antigens and antibodies as recognition units and used Mn-ZnS RTP QDs as sensing materials to specifically bind with MC-LR. The structurally specific binding between the microcystin-LR antibody and MC-LR led to the aggregation of antibody-crosslinked QDs, and then the electrons of QDs would be transferred to the complex, leading to the phosphorescence quenching of QDs. The microcystin-LR antigen-antibody specific binding site was first analyzed. This phosphorescent immunosensor rapidly and sensitively detected microcystin-LR, with linear ranges of 0.2-1.5 μg L and 1.5-20 μg L and a detection limit of up to 0.024 μg L. Meanwhile, coexisting pollutants of microcystin-LR in water did not significantly interfere with microcystin-LR detection. The new sensor was applied to detect real water samples and showed high sensitivity and selectivity.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9063644 | PMC |
| http://dx.doi.org/10.1039/c9ra02141h | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Development of fluorescent-photothermal probe based on photoinduced energy transfer: A dual-readout immunosensor for the detection of illegal additive.
Biosens Bioelectron
April 2025
Key Laboratory for Biobased Materials and Energy of Ministry of Education, College of Materials and Energy, South China Agricultural University, Guangzhou, 510642, China; Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Gua
The development of advanced optical probes for point-of-care testing holds great importance in the field of diagnostic technologies. This study focused on the synthesis of a probe featuring both fluorescent and photothermal responses with single excitation wavelength, which was achieved through the combination of oxidized camellia oleifera shell powder (OC) and Prussian blue nanoparticles (PBNPs). Notably, OC derived from the direct processing of raw material showed fluorescent and phosphorescent emissions simultaneously, and the positions of the two peaks overlapped with the absorbance range of PBNPs, making the fluorescent and phosphorescent emissions of OC effectively quenched by PBNPs.
View Article and Find Full Text PDFPhosphorescent palladium-tetrabenzoporphyrin indicators for immunosensing of small molecules with a novel optical device.
Talanta
March 2021
Department of Biotechnology, Israel Institute for Biological Research, Ness Ziona, 7410001, Israel. Electronic address:
Small-molecule detection is important for many applications including clinical diagnostics, drug discovery, environmental screening, and food technology. Current techniques suffer from various limitations including cost, complex sample processing, massive instrumentation, and need for expertise. To overcome these limitations, a new optical immunosensing assay for the detection of small molecules was developed and assessed with the targets estrone (E1) and estradiol (E2).
View Article and Find Full Text PDFA simple and sensitive Mn-ZnS quantum dot room-temperature phosphorescent immunosensor for detecting microcystin-LR was developed. This sensor adopted antigens and antibodies as recognition units and used Mn-ZnS RTP QDs as sensing materials to specifically bind with MC-LR. The structurally specific binding between the microcystin-LR antibody and MC-LR led to the aggregation of antibody-crosslinked QDs, and then the electrons of QDs would be transferred to the complex, leading to the phosphorescence quenching of QDs.
View Article and Find Full Text PDFPhosphorescent porphyrin probes in biosensors and sensitive bioassays.
Biochem Soc Trans
February 2000
Biochemistry Department, National University of Ireland, Cork, Lee Maltings, Ireland.
Platinum(II) and palladium(II) complexes of porphyrins and related tetrapyrrolic pigments emit strong phosphorescence at room temperatures, which is characterized by long lifetimes falling into the sub-millisecond range and long-wave spectral characteristics. These features make the dyes useful as probes for a number of bioanalytical applications, particularly those employing time-resolved fluorescent detection. They can provide high sensitivity and selectivity, together with rather simple instrumental set-up.
View Article and Find Full Text PDF