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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9674743 | PMC |
| http://dx.doi.org/10.1007/s10875-022-01280-y | DOI Listing |
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Epigenetic mechanisms and next-gen editing platforms in hematology: From molecular basis to therapeutic frontiers.
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September 2025
Student Research Committee, Department of Hematology and Blood Banking, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.. Electronic address:
Epigenetic regulation is fundamental to hematopoiesis, influencing stem cell fate, lineage commitment, and the development of hematologic diseases. Recent technological innovations have transitioned from traditional genetic editing towards programmable, reversible epigenetic modulation without altering the DNA sequence. This review explores the evolution of epigenetic editing platforms, from zinc finger proteins and TALEs to the transformative CRISPR-dCas9 system, and introduces next-generation technologies leveraging dCas12, dCas13, and modular RNA-guided systems.
View Article and Find Full Text PDFSemiLT: A Multianchor Transfer Learning Method for Cross-Modality Cell Label Annotation from scRNA-seq to scATAC-seq.
Adv Sci (Weinh)
September 2025
School of Mathematics, Shandong University, Jinan, Shandong, 250100, China.
scATAC-seq enables the detailed exploration of epigenetic variations across various cell clusters, providing complementary insights to scRNA-seq. However, its extreme sparsity and high dimensionality pose significant challenges for cell type annotation. Transfer learning can extract key features from well-annotated data to assist in annotating target data, thereby improving annotation accuracy.
View Article and Find Full Text PDFGaucher disease type 1 is a lysosomal storage disorder caused by mutations that reduce glucocerebrosidase activity, leading to glycolipid buildup, particularly in macrophages. To develop a curative approach, we established a high-efficiency genome editing platform for human and murine hematopoietic stem-progenitor cells using CRISPR/Cas9, recombinant adeno-associated virus serotype 6. To enhance homology-directed DNA repair while minimizing genotoxicity, we incorporated a new 53BP1 inhibitor, a ubiquitin variant that promotes DNA end resection and significantly increases editing efficiency.
View Article and Find Full Text PDFCommunity-acquired respiratory virus infections in patients with haematological malignancies or undergoing haematopoietic cell transplantation: updated recommendations from the 10th European Conference on Infections in Leukaemia.
Lancet Infect Dis
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Department of Medical Biology, Faculty of Health Sciences, UiT The Arctic University of Tromsø, Tromsø 9027, Norway; Transplantation & Clinical Virology, Department of Biomedicine, University of Basel, Basel, Switzerland. Electronic address:
To update recommendations of the 4th European Conference on Infections in Leukaemia (ECIL-4) on community-acquired respiratory virus (CARV) infections published in 2013, we reviewed publications from between Jan 1, 2014, and June 30, 2024 on adenovirus, bocavirus, coronavirus, influenzavirus, metapneumovirus, parainfluenzavirus, respiratory syncytial virus (RSV), and rhinovirus in patients with haematological malignancies or undergoing haematopoietic cell transplantation (HCT), or both. In the current ECIL recommendations (ECIL-10), we outline a common approach to infection control, laboratory testing, and diagnosis for all CARVs (including SARS-CoV-2) and specific management and deferral strategies for CARVs other than SARS-CoV-2. For influenzavirus, seasonal inactivated-vaccines and early antivirals are recommended, whereas routine antiviral prophylaxis is discouraged for immunocompromised patients.
View Article and Find Full Text PDFGermline and somatic variants in DNMT3A and other clonal haematopoiesis of indeterminate potential genes contribute to pulmonary arterial hypertension.
Eur Heart J
August 2025
Department of Medicine, Queen's University, Biosciences Complex Room 1520, 116 Barrie Street, Kingston, Ontario, Canada K7L 3N6.
Background And Aims: Multiple germline gene variants promote familial and idiopathic pulmonary arterial hypertension (PAH); however, none are consistently identified in associated PAH with connective tissue disease (APAH-CTD). Moreover, the role of somatic variants in genes mediating clonal haematopoiesis of indeterminate potential (CHIP) in PAH is unknown. Here, somatic and germline DNMT3A variants and CHIP gene variants in PAH were evaluated.
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