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ABA receptor agonists capable of improving plant performance under drought conditions have been described during the last years. However, monocot and eudicot plant species respond differently to various agonists. Here, we provide a detailed methodology to evaluate the anti-transpirant activity of ABA receptor agonists in both monocot and eudicot plant species using infrared imaging and image data analysis. Plant growth conditions, chemical application, and infrared image analysis are explained in detail to evaluate the anti-transpirant activity of ABA receptor agonists in the eudicot model Arabidopsis thaliana and in the C4-monocot model Setaria viridis.
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http://dx.doi.org/10.1007/978-1-0716-2297-1_16 | DOI Listing |
Physiol Plant
September 2025
College of Natural Resource and Environment, Northwest A&F University, Yangling, Shaanxi, China.
Nitrogen (N) is essential for plant growth, but excessive fertilizer use decreases nitrogen use efficiency (NUE) and raises environmental concerns. This study investigated the effect of exogenous abscisic acid (ABA; 50 μM) application on rapeseed (Brassica napus L.) plants under hydroponic conditions with high (7.
View Article and Find Full Text PDFPlants (Basel)
August 2025
Xinjiang Production & Construction Corps Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin, Aral 843300, China.
Abscisic acid (ABA) is a key phytohormone involved in regulating plant growth and responses to environmental stress. As receptors of ABA, pyrabactin resistance 1 (PYR)/PYR1-like (PYL) proteins play a central role in initiating ABA signal transduction. In this study, a total of 30 genes were identified and classified into three sub-families (PYL I-III) in the pan-genome of 17 species, through phylogenetic analysis.
View Article and Find Full Text PDFPlant Physiol
August 2025
State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, P. R. China.
Apple Valsa canker, caused by the ascomycete fungus Valsa mali, is a severe disease threatening apple (Malus domestica) production, particularly in East Asia. The pH at the infection site decreases from 6.0 to around 3.
View Article and Find Full Text PDFPlant Cell Physiol
August 2025
Institute of Plant Science and Resources, Okayama University, Kurashiki, Okayama, 710-0046, Japan.
Abscisic acid (ABA) signaling in stomatal guard cells is crucial for plants to cope with abiotic stress condition. Pyrabactin is a synthetic agonist of ABA that has a selective affinity to limited isoforms of ABA receptors. Here we investigated the differential utilization of downstream signaling events in guard cell ABA signaling under specific receptor isoforms taking advantage of pyrabactin affinity.
View Article and Find Full Text PDFJ Plant Physiol
September 2025
Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, Potsdam-Golm, Germany. Electronic address: