Engineering Novel ()-Selective Transaminase for Efficient Symmetric Synthesis of d-Alanine.

d-Alanine belongs to nonessential amino acids that have diverse applications in the fields of food and health care. ()-transaminase [()-TA]-catalyzed asymmetric amination of pyruvate is a feasible alternative for the synthesis of d-alanine, but low catalytic efficiency and thermostability limit enzymatic utilization. In this work, several potential ()-TAs were discovered using NCBI database mining synchronously with enzymatic structure-function analysis, among which Capronia epimyces TA (CeTA) showed the highest activity for amination of pyruvate using ()-α-methylbenzylamine as the donor. Furthermore, enzymatic residues surrounding a large catalysis pocket were subjected to saturation and combinatorial mutagenesis, and positive mutant F113T showed dramatic improvement in activity and thermostability. Molecular modeling indicated that the substitution of phenylalanine with threonine afforded alleviation of steric hindrance in the pocket and induced formation of additional hydrogen bonds with neighboring residues. Finally, using recombinant cells containing F113T as a biocatalyst, the conversion yield of amination of 100 mM pyruvate to d-alanine achieved up to 95.2%, which seemed to be the highest level in the literature regarding synthesis of d-alanine using TAs. The inherent characteristics rendered CeTA F113T a promising platform for efficient preparation of d-alanine operating with high productivity. d-Alanine is an important compound with many valuable applications. Its asymmetric synthesis employing ()-ω-TA is considered an attractive choice. According to the stereoselectivity, ω-TAs have either ()- or ()-enantiopreference. There has been a variety of literature regarding screening, characterizing, and molecular modification of ()-ω-TAs; in contrast, the research about ()-ω-TA has lagged behind. In this work, we identify several ()-ω-TAs and succeeded in creating mutant F113T, which showed not only better efficiency toward pyruvate but also higher thermostability compared with the original enzyme. The obtained original enzymes and positive mutants displayed important application value for pushing symmetric synthesis of d-alanine to a higher level.