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The emergence of antimicrobial-resistant has become a grave concern worldwide. In this study, 95 strains of isolated from stool samples were collected from Busan, South Korea to characterize their antimicrobial susceptibility, enterotoxin genes, and molecular typing using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and random amplification of polymorphic DNA (RAPD) assay. Only two strains showed no drug resistance, whereas resistance to three or more antibiotics was observed in 87.4% of strains. Ampicillin resistance was the most common at 90% and all strains were susceptible to vancomycin. The distribution of enterotoxin genes encoded in isolates was sea (32.6%), sec (11.6%), seg (19%), sea & sec (2.1%), and sec & seg (34.7%). Molecular typing using both MALDI-TOF MS and RAPD indicated that exhibited diverse clonal lineages and no correlations were observed among the profiling of enterotoxin, MALDI-TOF MS, and RAPD. This investigation provides useful information on foodborne pathogenic that has a significant public health impact in South Korea.
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http://dx.doi.org/10.3390/microorganisms10030642 | DOI Listing |
APMIS
September 2025
Laboratory of Parasitology, Department of Bacteria, Parasites and Fungi, Infectious Disease Preparedness, Statens Serum Institut, Copenhagen, Denmark.
Clinical microbiology involves the detection and differentiation of primarily bacteria, viruses, parasites and fungi in patients with infections. Billions of people may be colonised by one or more species of common luminal intestinal parasitic protists (CLIPPs) that are often detected in clinical microbiology laboratories; still, our knowledge on these organisms' impact on global health is very limited. The genera Blastocystis, Dientamoeba, Entamoeba, Endolimax and Iodamoeba comprise CLIPPs species, the life cycles of which, as opposed to single-celled pathogenic intestinal parasites (e.
View Article and Find Full Text PDFCan J Infect Dis Med Microbiol
August 2025
Department of Laboratory Medicine, Daejeon Eulji Medical Center, Eulji University, Daejeon, Republic of Korea.
This study aims to determine the molecular features and antimicrobial resistance of (Group B streptococcus, GBS) causing invasive and noninvasive infections in Korean adults. Sequence type (ST), capsular serotype, pilus island typing, and antimicrobial susceptibility were analyzed for GBS isolates obtained at a hospital laboratory that processed the primary clinical specimens collected from Korean adults between 2021 and 2024. Among the 90 isolates, Serotype VIII (34.
View Article and Find Full Text PDFFront Cell Infect Microbiol
September 2025
Unit for Environmental Sciences and Management, North-West University, Potchefstroom, South Africa.
Introduction: are commonly found in intramammary infections associated with bovine subclinical mastitis in dairy cattle, yet their genomic diversity and antimicrobial resistance dynamics remain poorly characterized, particularly in African settings.
Methods: This study presents a comparative genomic analysis of 17 isolates from South Africa, including five newly sequenced bovine mastitis strains and twelve porcine-derived genomes retrieved from GenBank. analysis using multilocus sequence typing (MLST), virulence genes, antibiotic resistance genes and plasmids replicon types were used to characterise these isolates.
NAR Genom Bioinform
September 2025
Research Group for Genomic Epidemiology, National Food Institute, Technical University of Denmark, 2800 Kgs. Lyngby, Denmark.
Advances in Oxford Nanopore Technologies (ONT) with the introduction of the r10.4.1 flow cell have reduced the sequencing error rates to <1%.
View Article and Find Full Text PDFGlob Chang Biol
September 2025
British Antarctic Survey, Cambridge, UK.
To date, environmental conditions have been enough to act as an effective barrier to prevent non-indigenous species from arriving and establishing in Arctic Canada. However, rapidly changing climatic conditions are creating more suitable habitats for non-indigenous species to potentially establish and become invasive. Concurrently, shipping traffic in parts of Arctic Canada has increased by over 250% since 1990, providing an effective vector for transporting non-indigenous species to the region.
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