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Article Abstract
, the causal agent of papaya dieback disease, is a devastating pathogen that has caused a tremendous decrease in Malaysian papaya export and affected papaya crops in neighbouring countries. A few studies on bacterial species capable of suppressing have been reported, but the availability of antagonistic fungi remains unknown. In this study, mycelial suspensions from five rhizospheric isolates of Malaysian origin were found to exhibit notable antagonisms against during co-cultivation. We further characterised three isolates, UKM-M-UW RA5, UKM-M-UW RA6, and UKM-M-UW RA3a, that showed significant growth inhibition zones on plate-based inhibition assays. A study of the genomes of the three strains through a combination of Oxford nanopore and Illumina sequencing technologies highlighted potential secondary metabolite pathways that might underpin their antimicrobial properties. Based on these findings, the fungal isolates are proven to be useful as potential biological control agents against and the genomic data opens possibilities to further explore the underlying molecular mechanisms behind their antimicrobial activity, with potential synthetic biology applications.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8949440 | PMC |
| http://dx.doi.org/10.3390/jof8030246 | DOI Listing |
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Article Synopsis
- The text discusses a pathogen causing papaya dieback disease, significantly impacting Malaysia's papaya exports and neighboring countries' crops.
- The study found five Malaysian fungal isolates showing antagonism against the pathogen, with three exhibiting notable growth inhibition.
- Genomic analysis revealed potential pathways for antimicrobial properties, suggesting these fungi could be developed as biological control agents and explored for synthetic biology applications.
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