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In the present study, ground ivy was harvested from different natural habitats in Croatia and subjected to screening analysis for nutritional and bioactive composition. To achieve maximum recovery of phenolic compounds, different extraction techniques were investigated-heat-assisted (HAE), microwave-assisted (MAE) and subcritical water (SWE) extraction. Prepared extracts were analysed by spectrophotometric methods, LC-MS/MS and HPLC-PAD methodologies. Results regarding nutritive analyses, conducted using standard AOAC methods, showed the abundance of samples in terms of insoluble dietary fibre, protein, calcium and potassium, while rutin, chlorogenic, cryptochlorogenic, caffeic and rosmarinic acid were the most dominant phenolic compounds. In addition, LC-MS/MS analysis revealed the presence of apigenin and luteolin in glycosylated form. Maximum recovery of target phenolic compounds was achieved with MAE, while SWE led to the formation of new antioxidants, which is commonly known as neoformation. Moreover, efficient prediction of phenolic composition of prepared extracts was achieved using NIR spectroscopy combined with ANN modelling.
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http://dx.doi.org/10.3390/foods11050658 | DOI Listing |
PLoS One
September 2025
Misión Biológica de Galicia (MBG-CSIC), Pontevedra, Galicia, Spain.
Specialized plant metabolism, particularly phenolic compound production, contributes significantly to the functioning and resilience of mountain ecosystems. Livestock grazing can influence phenolic production, with its effects varying depending on microclimatic factors and soil conditions. Despite the ecological significance of this process, the impact of livestock grazing on phenolic production in alpine plants remains insufficiently explored.
View Article and Find Full Text PDFPlant Foods Hum Nutr
September 2025
Graduate School of Food and Nutritional Sciences, Toyo University, 48-1, Oka, 351-8501, Asaka, Saitama, Japan.
Pea shoots (Pisum sativum) are well known to have nutritional benefits when consumed raw; however, the effects of home cooking on their bioactive compounds remain unclear. Therefore, we investigated how different cooking methods affect the antioxidant activity and stability of antioxidants. Our evaluation revealed that antioxidant activity is preserved by steaming but significantly reduced by microwaving and boiling, which also causes weight loss during cooking.
View Article and Find Full Text PDFAppl Biochem Biotechnol
September 2025
Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, Qassim University, 51452, Qassim, Saudi Arabia.
Viruses are minuscule entities that cannot survive independently without a Living host. Pathogenic viruses pose a significant threat to global health, resulting annually in the deaths of thousands of people. Recent studies indicate that medicinal plants may serve as an effective source of sustainable natural antiviral agents.
View Article and Find Full Text PDFFEMS Yeast Res
September 2025
Enology and Fermentation Biotechnology Area, Department of Science and Food Technology. Faculty of Chemistry, Universidad de la Republica. Montevideo, Uruguay.
Hanseniaspora species are among the most prevalent yeasts found on grapes and other fruits, with a growing role in wine fermentation due to their distinctive metabolic profiles. This review focuses on the functional divergence within the genus, particularly between the fast-evolving fruit clade and the slow-evolving fermentation clade. While species in the fruit clade often exhibit limited fermentation capacity with interesting enzymatic activity, members of the fermentation clade-especially H.
View Article and Find Full Text PDFChemSusChem
September 2025
Great Lakes Bioenergy Research Center, Wisconsin Energy Institute, University of Wisconsin-Madison, Madison, WI 53706, USA.
Spent liquors of biomass pretreatment provide a source for renewable chemical production. These liquors require treatment before being discharged; otherwise, they negatively impact the environment. Herein, spent liquors from aqueous ammonia pretreatment of poplar wood are characterized for phenolic content via liquid chromatography-mass spectrometry and nuclear magnetic resonance spectroscopy.
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