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Article Abstract
Human immune system (HIS) mice provide a valuable platform to investigate and modulate human hematopoiesis development . Here, we describe detailed protocols for the construction of HIS mice, modulation of human hematopoiesis using hydrodynamic injection of plasmids encoding cytokines of interest, and flow cytometry analysis of humanization levels and human immune subsets. This approach can be easily applied to screen or verify factors that regulate human hematopoiesis and immune system. For complete details on the use and execution of this protocol, please refer to Cardoso et al. (2021) and Li et al. (2017).
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8899045 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2022.101217 | DOI Listing |
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