Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
The venom duct transcriptomes and proteomes of the cryptic cone snail species and were obtained and compared. The most abundant and shared conotoxin precursor superfamilies in both species were M, O1, and O2. Additionally, three new putative conotoxin precursor superfamilies (Virro01-03) with cysteine pattern types VI/VII and XVI were identified. The most expressed conotoxin precursor superfamilies were SF-mi2 and M in , and Cerm03 and M in . Up to 16 conotoxin precursor superfamilies and hormones were differentially expressed between both species, and clustered into two distinct sets, which could represent adaptations of each species to different diets. Finally, we predicted, with machine learning algorithms, the 3D structure model of selected venom proteins including the differentially expressed Cerm03 and SF-mi2, an insulin type 3, a GVIA-like conotoxin, and an ortholog to the ω-conotoxin MVIIA (Ziconotide).
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8875821 | PMC |
| http://dx.doi.org/10.3390/md20020149 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Molecular Insights into the Marine Gastropod : Transcriptomic and Proteopeptidomic Approaches Reveal Polypeptides with Putative Therapeutic Potential.
Int J Mol Sci
April 2025
Department of Biological and Environmental Sciences, Bioscience Institute, São Paulo State University (UNESP), Sao Vicente 11330-900, SP, Brazil.
The marine environment is a rich source of new biotechnologies and products. Bottom trawling for shrimp species such as and leads to the unintentional capture of non-target species, known as bycatch, which includes a variety of marine life that are often discarded without economic value. A common bycatch species on the southeast coast of Brazil is (), a carnivorous gastropod that feeds mainly on bivalves.
View Article and Find Full Text PDFStructural characterisation of a cysteine-rich conotoxin, sigma(σ)S-GVIIIA, extracted from the defensive venom of the marine cone snail Conus geographus.
Biochem J
April 2025
Australian Institute of Tropical Health and Medicine, James Cook University, Cairns, QLD, Australia.
The activity of the serotonin type 3 (5-HT3) receptor is associated with neurodegenerative, inflammatory and metabolic diseases, neuropsychiatric disorders, and cancer. Structural analysis of modulators of this receptor is likely to aid in future medicinal chemistry studies aimed at developing lead molecules targeting this receptor. Here we report the structure of a cone snail venom peptide that was purified from the crude venom of Conus geographus and shown to be an antagonist of the 5-HT3 receptor more than 25 years ago, sigma(σ)GVIIIA.
View Article and Find Full Text PDFRedox-Active Conopeptide Li520 Has Evolved to Catalyze Oxidative Folding of Conotoxins.
ACS Omega
September 2024
Department of Chemistry, School of Chemical Sciences, Central University of Karnataka, Kalaburagi 585367, Karnataka, India.
Article Synopsis
- The study investigates the evolution of the miniature conopeptide Li520, highlighting its disulfide isomerase activity by examining its structure and gene sequence.
- The synthesized peptides, including Li520 and its variants, show varying disulfide reduction potentials, indicating their effectiveness in protein folding and disulfide exchange reactions.
- Findings suggest that Li520's specific amino acid composition plays a crucial role in its functionality, and the peptide's evolution may aid in developing therapeutic peptides.
Proteomic analysis of the venom of Conus flavidus from Red Sea reveals potential pharmacological applications.
J Genet Eng Biotechnol
June 2024
Zoology Department, Faculty of Science, Al-Azhar University, Assiut Branch 71524, Assuit, Egypt; Department of Biomedical Sciences, College of Clinical Pharmacy, King Faisal University, 31982, Saudi Arabia.
Background: Venomous marine cone snails produce unique neurotoxins called conopeptides or conotoxins, which are valuable for research and drug discovery. Characterizing Conus venom is important, especially for poorly studied species, as these tiny and steady molecules have considerable potential as research tools for detecting new pharmacological applications. In this study, a worm-hunting cone snail, Conus flavidus inhabiting the Red Sea coast were collected, dissected and the venom gland extraction was subjected to proteomic analysis to define the venom composition, and confirm the functional structure of conopeptides.
View Article and Find Full Text PDFCysteine-free cone snail venom peptides: Classification of precursor proteins and identification of mature peptides.
J Pept Sci
April 2024
National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore, India.
The cysteine-free acyclic peptides present in marine cone snail venom have been much less investigated than their disulfide bonded counterparts. Precursor protein sequences derived from transcriptomic data, together with mass spectrometric fragmentation patterns for peptides present in venom duct tissue extracts, permit the identification of mature peptides. Twelve distinct gene superfamiles have been identified with precursor lengths between 64 and 158 residues.
View Article and Find Full Text PDF