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Visualizing dynamic cellular behaviors using live imaging is critical to the study of cell movement and to the study of cellular and embryonic polarity. Similarly, live imaging can be vital to elucidating the pathology of genetic disorders and diseases. Model systems such as zebrafish, whose in vivo development is accessible to both the microscope and genetic manipulation, are particularly well-suited to the use of live imaging. Here we describe an overall approach to conducting live-imaging experiments with a specific emphasis on investigating cell movements during the early stages of heart development in zebrafish.
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http://dx.doi.org/10.1007/978-1-0716-2035-9_9 | DOI Listing |
Ann Intern Med
September 2025
Department of Medicine, St Michael's Hospital, University of Toronto, Toronto, Ontario, Canada (J.G.R.).
Background: Animal studies show ovarian follicle damage and mutagenesis after ionizing radiation exposure. Computed tomography (CT) imaging is commonly done outside pregnancy, but risks to future pregnancy are unknown.
Objective: To evaluate the risk for spontaneous pregnancy loss and congenital anomalies in offspring of women exposed to CT ionizing radiation before conception.
Proc Natl Acad Sci U S A
September 2025
Molecular Imaging Program at Stanford, Department of Radiology, School of Medicine, Stanford University, Palo Alto, CA 94304.
The biophysical properties of single cells are crucial for understanding cellular function and behavior in biology and medicine. However, precise manipulation of cells in 3-D microfluidic environments remains challenging, particularly for heterogeneous populations. Here, we present "Electro-LEV," a unique platform integrating electromagnetic and magnetic levitation principles for dynamic 3-D control of cell position during separation.
View Article and Find Full Text PDFJ Vis Exp
August 2025
Institut de recherches cliniques de Montréal (IRCM); Programmes de biologie moléculaire, Université de Montréal; Département de Médecine, Université de Montréal;
Embryonic tissue growth and patterning are largely controlled by signals exchanged locally between cell populations within the tissues themselves. Cytonemes are a type of signaling filopodia first identified in Drosophila that connect and mediate exchange between signal-producing and signal-receiving cells. In the developing Drosophila wing imaginal disc, cytonemes are involved in signal exchange between distinct populations of cells within the disc proper (DP) epithelium, which will form the adult wing, as well as between DP cells and cells in adjacent disc-associated tissues.
View Article and Find Full Text PDFJ Biomed Opt
December 2025
University of Toronto, Department of Medical Biophysics, Temerty Faculty of Medicine, Toronto, Ontario, Canada.
Significance: Tumor tissues exhibit contrast with healthy tissue in circular degree of polarization (DOP) images via higher magnitude circular DOP values and increased helicity-flipping. This phenomenon may enable polarimetric tumor detection and surgical/procedural guidance applications.
Aim: Depolarization metrics have been shown to exhibit differential responses to healthy and cancer tissue, whereby tumor tissues tend to induce less depolarization; however, the understanding of this depolarization-based contrast remains limited.
MicroPubl Biol
August 2025
Department of Basic Science, Graduate School of Arts and Sciences, The University of Tokyo, Tokyo, Japan.
Because of its good spectral separation from green (GFP) and red (RFP) fluorescent proteins, blue fluorescent protein (BFP) is essential for multicolor live cell imaging. However, the commonly used bright mTagBFP2 strongly perturbs the cellular localization of Lifeact, an F-actin marker. As an alternative, we tested the expression of Electra2 in .
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