Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
The skeletal muscle system is the major organ associated with movement of the body. Myogenesis and regeneration induced post-injury contribute to muscle formation and maintenance. Here, we provide detailed protocol for the accelerated repair of injured skeletal muscles and generation of hypertrophic muscle fibers. This protocol includes cardiotoxin induced muscle injury and also describes isolation of satellite cells from skeletal muscle tissues of mice. This protocol can be used to study the mechanisms associated with accelerated muscle repair and hypertrophy. For complete details on the use and execution of this protocol, please refer to Ray et al. (2021).
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8792418 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2021.101111 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Possible involvement of p60-S6K1 in accelerating RPS6 phosphorylation for rapid recovery from skeletal muscle disuse atrophy.
Lab Anim Res
September 2025
Department of Pathology, Faculty of Medicine, Kindai University, 377-2 Ohno-Higashi, Osaka-Sayama, Osaka, 589-8511, Japan.
Background: Stroke-prone spontaneously hypertensive rats (SHRSP) exhibit slow-twitch muscle-specific hypotrophy compared with normotensive Wistar-Kyoto rats (WKY). Because slow-twitch muscles are prone to disuse atrophy, SHRSP may experience both disuse atrophy and impaired recovery from it. This study investigated the response of SHRSP to disuse atrophy and subsequent recovery, using WKY as a control.
View Article and Find Full Text PDFMolecular subtypes of human skeletal muscle in cancer cachexia.
Nature
September 2025
Department of Laboratory Medicine and Pathology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta, Canada.
Cancer-associated muscle wasting is associated with poor clinical outcomes, but its underlying biology is largely uncharted in humans. Unbiased analysis of the RNAome (coding and non-coding RNAs) with unsupervised clustering using integrative non-negative matrix factorization provides a means of identifying distinct molecular subtypes and was applied here to muscle of patients with colorectal or pancreatic cancer. Rectus abdominis biopsies from 84 patients were profiled using high-throughput next-generation sequencing.
View Article and Find Full Text PDFAssessment of different preservation techniques for human skeletal muscle biopsy samples: A comparative method study on freeze-drying, RNAlater, and RNAlater-ICE.
Physiol Rep
September 2025
Department of Physiology, Nutrition and Biomechanics, Swedish School of Sport and Health Sciences, Stockholm, Sweden.
Human skeletal muscle comprises slow-twitch (type I) and fast-twitch (type II) fibers. Fiber type-specific analyses often require manual isolation of fibers, necessitating effective tissue preservation. While freeze-drying remains the standard, alternative preservation methods such as RNAlater and RNAlater-ICE are increasingly used.
View Article and Find Full Text PDFAssociation Between Body Composition and Obesity Phenotypes in Children and Adolescents.
Ann N Y Acad Sci
September 2025
Department of Epidemiology and Biostatistics, School of Public Health, Guangdong Pharmaceutical University, Guangzhou, China.
Assessment of influencing factors is critical for the management of different obesity phenotypes among children and adolescents. We investigated the association between body composition and metabolically unhealthy phenotypes independently or in an interaction with physical activity or sleep, among 7572 children and adolescents with normal weight or overweight/obesity from Guangzhou, China. High body fat percentage (BF%), trunk-to-limb fat ratio (T/L), waist-to-height ratio (WHtR), low soft lean mass percentage (SLM%), and appendicular skeletal muscle percentage (ASM%) were all associated with increased risk of metabolically unhealthy overweight/obesity (MUO) (odds ratios ranging from 1.
View Article and Find Full Text PDFAssociations of body mass index, waist circumference, and weight-adjusted waist index with body composition and biomarkers: A multinational pooled analysis.
Am J Clin Nutr
September 2025
Interdisciplinary Program in Precision Public Health, Department of Public Health Sciences, Graduate School of Korea University, Seoul, Republic of Korea; Department of Health Policy and Management, College of Health Sciences, Korea University, Seoul, Republic of Korea. Electronic address: hannahoh@
Background: The widely-used anthropometric indices, such as body mass index (BMI) and waist circumference (WC), have limitations in their use as indicators of body composition. Recent studies proposed weight-adjusted waist index (WWI=WC/√(body weight)) as an alternative index for body composition but it is unclear whether WWI reflects body composition in different racial/ethnic groups.
Objective: We examined the associations of WWI, BMI, and WC with dual-energy x-ray absorptiometry (DEXA)-measured body composition, biomarkers (fasting blood glucose, HDL-cholestrol, LDL-cholestrol, triglyceride), and handgrip strength.