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Yak, a unique bovine species on the Qinghai-Tibetan Plateau, has provided pastoralists with the basic materials of production and life for thousands of years. Existing literature showed that yak meat is of high nutritional value for humans whereas the growth performance is relatively low. As it has been demonstrated, lysine and methionine are the two key limiting amino acids in ruminants. Therefore, this study was conducted to investigate the effect of supplementary levels of rumen-protected lysine (RPL) and methionine (RPM) on growth performance, carcass traits, and meat quality in feedlot yaks. Thirty-two male yaks, with initial body weight (IBW) of 237.5 ± 13.99 kg were randomly assigned to four dietary treatments ( = 8), including control group (CON; basal diet without adding rumen-protected amino acid (RPAA)), low RPAA supplementation group (Group Low; basal diet supplemented with RPL (30.0 g/animal/day) and RPM (7.5 g/animal/day)), medium RPAA supplementation group (Group Medium; basal diet supplemented with RPL (50.0 g/animal/day) and RPM (12.5 g/animal/day)), and high RPAA supplementation group (Group High; basal diet supplemented with RPL (70.0 g/animal/day) and RPM (17.5 g/animal/day)). The average daily gain was increased linearly ( < 0.001) and quadratically ( < 0.01) while feed to gain ratio was decreased linearly ( < 0.001) and quadratically ( < 0.001) with the increasing RPAA supplementation, and the average daily gain was higher while feed to gain ratio was lower in RPAA than CON ( < 0.001). Meat color L* values and b* were decreased linearly ( < 0.01) with the increase of RPAA supplementation, and b* was lower in RPAA than CON ( < 0.05). Meat color a* value was increased linearly ( < 0.05) with the increasing RPAA supplementation, and a* was higher in RPAA than CON ( < 0.05). The 24 h drip loss and shear force were decreased quadratically ( < 0.01) with the increasing RPAA supplementation, and drip loss and shear force values were lower in RPAA than CON ( < 0.05). The glycine concentration in (LD) increased linearly ( < 0.05) with the increasing RPAA supplementation. These results demonstrated that both the growth performance and meat quality characteristics were improved in feedlot yaks as an effect of the dietary supplementation with RPL and RPM, and the medium supplementary level is recommended, since it showed the most promising results.
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http://dx.doi.org/10.3390/ani11123384 | DOI Listing |
Animals (Basel)
August 2025
Department of Animal Science, Texas A&M University, College Station, TX 77843, USA.
Low fertility limits reproductive efficiency in cattle. This study was conducted with multiparous Brangus cows receiving dietary supplementation with or without L-citrulline [Cit; an immediate precursor of L-arginine (Arg)]. During the entire experimental period, cows grazed green pasture and had free access to drinking water and mineral blocks.
View Article and Find Full Text PDFJ Dairy Sci
January 2025
The Pennsylvania State University, University Park, PA 16802. Electronic address:
The objective of this experiment was to estimate the bioavailability (BA) of rumen-protected (RP) His, RPLys, and 2 RPMet products using 3 in vivo methods: area under the curve (AUC), plasma dose-response (PDR), and fecal free AA (FFAA) methods. We used 8 rumen-cannulated cows in a replicated 4 × 4 Latin square experiment with 16-d periods. Treatments were (1) abomasal infusion of water (control), (2) abomasal infusion of free His, Lys, and Met (FAA), (3) administration of RPHis + RPLys + RPMet1 (rumen-protected methionine protected with ethyl cellulose; RPAA1), and (4) administration of RPHis + RPLys + RPMet2 (rumen-protected methionine protected with a pH-sensitive polymer; RPAA2).
View Article and Find Full Text PDFJ Dairy Sci
September 2024
Animal Nutrition Group, Wageningen University and Research, 6700 AH Wageningen, the Netherlands.
Animals (Basel)
February 2023
College of Animal Science and Technology, Hebei Agricultural University, Baoding 071000, China.
The aim of this study was to investigate the effect of low-protein diets supplemented with rumen-protected lysine (RPLys) and methionine (RPMet) on growth performance, rumen fermentation, blood biochemical parameters, nitrogen metabolism, and gene expression related to N metabolism in the liver of Holstein bulls. Thirty-six healthy and disease-free Holstein bulls with a similar body weight (BW) (424 ± 15 kg, 13 months old) were selected. According to their BW, they were randomly divided into three groups with 12 bulls in each group in a completely randomized design.
View Article and Find Full Text PDFFront Vet Sci
March 2022
State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.
The objectives of this study were to evaluate the effects of dietary supplementation of ruminally protected lysine (RPL), or methionine (RPM), and their combination (RPML) on the production efficiency of transition cows. A total of 120 pre-partum multiparous Holstein cows were assigned to four treatments based on previous lactation milk production, days (d) of pregnancy, lactation, and body condition score (BCS). Cows were fed a basal diet [pre-calving: 1.
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