Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
The Rapid Alkalinization Factor (RALF) is a plant hormone peptide that inhibits proton transport causing alkalinization of the extracellular media. To detect the alkalinization response elicited by RALF peptides in root cells, seedlings are carefully transferred to a gel containing the pH-sensitive indicator bromocresol purple, treated with the peptide and photographed after 30 min. Herein the protocol is optimized for evaluation of exogenous treatment, described in detail and expected results are presented.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8275321 | PMC |
| http://dx.doi.org/10.21769/BioProtoc.2796 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Tacrolimus-Loaded Superabsorbent Hydrogels: Formulation, Evaluation, and Therapeutic Potential.
ACS Omega
September 2025
Department of Pharmaceutical Sciences, College of Pharmacy, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia.
Tacrolimus belongs to the BCS class-II drug family and exhibits poor water solubility, which leads to poor bioavailability. Furthermore, since tacrolimus is an immunosuppressant, it is essential to maintain its therapeutic concentration for a greater period of time to confirm its effectiveness against transplant rejection. Therefore, to achieve the objective of the sustained release of the drug with a suitable amount of entrapment efficiency, pH-sensitive tacrolimus-loaded superabsorbent hydrogels using chitosan have been prepared.
View Article and Find Full Text PDFColorimetric pH-responsive nanofibrous hydrogels for monitoring of wound infection.
J Mater Chem B
August 2025
Laboratory of Advanced Fibers, Empa, Swiss Federal Laboratories for Materials Science and Technology, Lerchenfeldstrasse 5, 9014 St. Gallen, Switzerland.
Effective wound management is crucial for improving patient outcomes, particularly through timely detection of infections and monitoring of wound conditions. Addressing this critical need, this research develops nanofibrous hydrogels integrated with an indicator dye for real-time monitoring of wounds a colorimetric method. A new double network nanofibrous hydrogels based on polyvinyl alcohol (PVA) and a phosphine oxide-based pH-sensitive hydrogel (namely PVA/Gel-A) with improved properties were produced through electrospinning.
View Article and Find Full Text PDFGreen Smart Packaging Film Based on Kappa-Carrageenan/Poly(Vinyl Alcohol) Enriched with Mexican Petunia Anthocyanins for Visual Detection of Food Freshness.
ACS Omega
August 2025
Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Burapha University, Chonburi 20131, Thailand.
This research presents a novel approach for the development of environmentally friendly and pH-sensitive films composed of kappa-carrageenan and poly-(vinyl alcohol), designed to facilitate real-time monitoring of food freshness. Sol-gel cross-linked films, enriched with natural anthocyanins extracted from Mexican petunia flowers, were prepared using an aqueous solution casting method. The formation of siloxane cross-linking and the successful incorporation of anthocyanins were confirmed by ATR-FTIR spectroscopy.
View Article and Find Full Text PDFMucilage-based nanocarriers for targeted cancer therapy-design, functionalization, and therapeutic potential.
Drug Dev Ind Pharm
August 2025
Department of Chemistry, Egerton University, Njoro, Kenya.
Objective: To provide a comprehensive evaluation of mucilage-based nanocarriers as emerging platforms for targeted cancer therapy, focusing on their design, functionalization, and therapeutic potential.
Significance: Mucilage, a plant-derived biopolymer composed of natural polysaccharides, possesses inherent biocompatibility, biodegradability, and unique physicochemical characteristics such as high-water retention, gel-forming ability, and stimuli-responsiveness. These properties position mucilage as an ideal material for controlled drug delivery in oncology, offering potential improvements over conventional nanocarriers.
Mallotus philippensis Extract-Infused Locust Bean Gum-Based Ternary Hydrogel: A Green Fabrication Approach for the Controlled Release of Levofloxacin.
Macromol Biosci
July 2025
Institute of Polymer Materials, Department of Material Science and Engineering, Faculty of Engineering, Friedrich-Alexander University Erlangen-Nuremberg, Martensstrasse 7, Erlangen, Bavaria, Germany.
Smart and responsive drug delivery systems are key to next-generation biomedical therapies, offering precision and targeted action. This study reports the development of a novel biodegradable, pH-sensitive, and highly swellable hydrogel composed of locust bean gum, polyvinylpyrrolidone, chitosan, and (3-aminopropyl)triethoxysilane (APTES) as a crosslinker, incorporating Mallotus philippensis (MP) extract to enhance bioactivity. Structural, thermal, and morphological properties were characterized by FT-IR, TGA, and SEM.
View Article and Find Full Text PDF