Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Early events in retrovirus transmission are determined by interactions between incoming viruses and frontline cells near entry sites. Despite their importance for retroviral pathogenesis, very little is known about these events. We developed a bioluminescence imaging (BLI)-guided multiscale imaging approach to study these events in vivo. Engineered murine leukemia reporter viruses allowed us to monitor individual stages of retrovirus life cycle including virus particle flow, virus entry into cells, infection and spread for retroorbital, subcutaneous, and oral routes. BLI permitted temporal tracking of orally administered retroviruses along the gastrointestinal tract as they traversed the lumen through Peyer's patches to reach the draining mesenteric sac. Importantly, capture and acquisition of lymph-, blood-, and milk-borne retroviruses spanning three routes was promoted by a common host factor, the I-type lectin CD169, expressed on sentinel macrophages. These results highlight how retroviruses co-opt the immune surveillance function of tissue-resident sentinel macrophages for establishing infection.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8298093 | PMC |
| http://dx.doi.org/10.7554/eLife.64179 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Preclinical Fluorescence-Guided Imaging Leveraging Surrounding Sentinel Tumor Microenvironment Identifies High-Risk Premalignant Pancreatic Lesions.
Clin Cancer Res
August 2025
The University of Texas MD Anderson Cancer Center, Houston, Tx, United States.
Purpose: With surgery being the only potential cure for pancreatic cancer, high-risk premalignant pancreatic lesions often go unnoticed by palpation or white light visualization, leading to recurrence. We asked whether near-infrared fluorescence imaging of tumor-associated inflammation could identify high-risk premalignant lesions, leveraging the tumor microenvironment (TME) as a sentinel of local disease and, thus, enhance surgery outcomes.
Experimental Design: Fluorescence-guided surgery was performed on genetically engineered mice (Ptf1a-Cre; LSL-KrasG12D/+; Smad4flox/flox [KSC]) at discrete stages of disease progression, histologically confirmed high-risk, premalignant lesions in postnatal mice to locally advanced pancreatic tumors in adults, using the imaging agent V-1520, a translocator protein (TSPO) ligand.
Gut commensal microbiota drive tailored macrophage responses.
Cell Rep
August 2025
Meinig School of Biomedical Engineering, Cornell University, Ithaca, NY, USA. Electronic address:
Macrophages serve as sentinels at the intestinal surface, responding to organismal cues to drive proinflammatory or tolerogenic responses. To date, studies of combinations of these cues do not fully capture the heterogeneity of macrophage responses. To address this gap, we performed multiplexed single-cell RNA sequencing on 74,476 human monocyte-derived macrophages following exposure to 15 bacteria, mostly commensals.
View Article and Find Full Text PDFEngineered nanomaterials and macrophages: Toxicity mechanisms and protective strategies.
Int Immunopharmacol
September 2025
State Key Laboratory of Oral Diseases & National Center for Stomatology & National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, Sichuan, China. Electronic address:
Engineered nanomaterials (ENMs) have gained extensive applications across diverse fields, yet their potential cytotoxicity, particularly toward the immune system, raises significant safety concerns. As critical sentinels of innate immunity, macrophages directly interact with ENMs, making them pivotal targets for nanotoxicity studies. This review systematically addresses the interplay between ENMs and macrophages to advance their safe therapeutic utilization.
View Article and Find Full Text PDFIFNγ-induced memory in human macrophages is not sustained by epigenetic changes but the durability of the cytokine itself.
bioRxiv
July 2025
Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, Los Angeles, CA, USA.
Macrophages, as key sentinel cells of the innate immune system, can retain memory of prior stimulus exposure. Interferon gamma (IFNγ) plays a central role in maintaining trained immunity and can induce potent memory in macrophages. Such memory is associated with the formation of enhancers that alter gene expression responses to subsequent stimuli.
View Article and Find Full Text PDFPlans within plans: post-transcriptional regulation governs macrophage responses.
Trends Immunol
August 2025
Department of Biochemistry, University of Colorado, Boulder, CO, 80303, USA; Howard Hughes Medical Institute, University of Colorado, Boulder, CO, 80303, USA. Electronic address:
Macrophages are sentinels and first responders of the innate immune system. By sensing danger signals, they initiate and amplify inflammatory and regenerative cascades to control appropriate responses to pathogens and tissue damage. Transcriptional activation of macrophage gene expression has been studied extensively, but macrophage responses also rely on regulation of mRNAs following transcription.
View Article and Find Full Text PDF