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http://dx.doi.org/10.1016/j.ymgmr.2021.100737 | DOI Listing |
Acc Chem Res
September 2025
Department of Pharmaceutical Chemistry and Small Molecule Discovery Center, University of California, San Francisco 94158, United States.
ConspectusProtein-protein interactions (PPIs) play a key role in homeostasis and are often dysregulated in disease. PPIs were traditionally considered "undruggable" due to their flat surfaces and disordered domains. Recently, the identification of PPI stabilizers, or molecular glues (MGs), compounds that bind cooperatively to PPI interfaces, has provided a new direction for the field.
View Article and Find Full Text PDFSci Adv
September 2025
Department of Biochemistry, University of Toronto, 661 University Ave., Toronto, Ontario M5G 1M1, Canada.
Host cells provide intracellular bacteria with protection from harsh environmental conditions and immune responses, but for many intracellular pathogens, this protection does not appear to be absolute as once thought. Bacteriophages that can kill bacteria inside host cells have been identified for pathogens including , , and species. Even in pathogens for which no stable phages have been isolated, such as , the presence of phage defense systems suggests phage susceptibility.
View Article and Find Full Text PDFAngew Chem Int Ed Engl
September 2025
Department of Chemistry, Emory University, 1515 Dickey Drive, Atlanta, GA, 30322, USA.
High-throughput experimentation (HTE), the miniaturization and parallelization of reactions, is a valuable tool for accelerating diverse compound library generation, optimizing reaction conditions, and enabling data collection for machine learning (ML) applications. When applied to organic synthesis and methodology, HTE still poses various challenges due to the diverse workflows and reagents required, motivating advancements in reaction design, execution, analysis, and data management. To address these limitations, cutting-edge technologies, automation, and artificial intelligence (AI) have been implemented to standardize protocols, enhance reproducibility, and improve efficiency.
View Article and Find Full Text PDFLancet Infect Dis
August 2025
Dale and Betty Bumpers Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA. Electronic address:
Background: The Pandemic Response Repository through Microbial and Immune Surveillance and Epidemiology (PREMISE) programme was established to translate knowledge gained from global immunoepidemiological surveillance into a better understanding of population-level dynamics of emerging and re-emerging infections, as well as into the discovery and development of biomedical countermeasures against potential pandemic threats. As proof of principle for this approach, we conducted a longitudinal immunoepidemiological study in children in the USA, focusing on enterovirus D68 (EV-D68) infection dynamics but also capturing surveillance of a broad array of other endemic respiratory pathogens. Serendipitously, our sampling spanned the lifting of widespread COVID-19 non-pharmaceutical interventions (NPIs) in 2022-23, following a unique period during which virus exposure markedly diminished.
View Article and Find Full Text PDFACS Infect Dis
August 2025
Department of Molecular Microbiology, A-LIFE, AIMMS, VU Amsterdam, De Boelelaan 1108, 1081 HZ Amsterdam, The Netherlands.
The highly impermeable cell envelope of Gram-negative bacteria is an important hurdle to the development of novel antibacterials. However, compounds that disrupt the integrity of the cell envelope can act as potent antibiotics by directly inhibiting cell growth and viability or by enhancing the penetration of other, larger antibiotics otherwise unable to pass this barrier. To identify such novel compounds, we used the European Lead Factory compound libraries to screen >500,000 small molecules for inducing the Rcs cell envelope stress response in .
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