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Article Abstract
Cell surface hydrophobicity (CSH) and adhesion are very important phenotypical traits for probiotics that confer them a competitive advantage for the resilience in the human gastrointestinal tract. This study was aimed to understand the effects over time of a 50 MPa hyperbaric treatment on the surface properties of 08 including CSH, autoaggregation, and adhesion (mucin layer and Caco-2 cells). Moreover, a link between the hurdle applied and the expression of genes involved in the general stress response ( and ) and adhesion processes ( and ) was evaluated. High pressure homogenization (HPH) at 50 MPa significantly increased the CSH percentage (H%), autoaggregation and adhesion on mucin of 08 cells compared with the untreated cells. Moreover, the hyperbaric hurdle induced an upregulation of the stress response genes and together with a down regulation of the and genes. Looking at the protein profile, HPH-treatment showed an increase in the number or intensity of protein bands at high and low molecular weights.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8193580 | PMC |
| http://dx.doi.org/10.3389/fmicb.2021.651711 | DOI Listing |
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