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Article Abstract
This protocol describes the use of the deterministic barcoding in tissue for spatial omics sequencing platform to construct a multi-omics atlas on fixed frozen tissue samples. This approach uses a microfluidic-based method to introduce combinatorial DNA oligo barcodes directly to the cells in a tissue section fixed on a glass slide. This technique does not directly resolve single cells but can achieve a near-single-cell resolution for spatial transcriptomics and spatial analysis of a targeted panel of proteins. For complete details on the use and execution of this protocol, please refer to Liu et al. (2020).
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8132129 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2021.100532 | DOI Listing |
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